Anti-Hu CD253 APC

• Description:

  Human CD253 / TRAIL (TNF-related apoptosis inducing ligand), also called Apo2, is a type II membrane protein from the TNF family. TRAIL is a cytotoxic protein which activates rapid apoptosis in tumor cells, but not in normal cells. TRAIL-induced apotosis, is achieved through binding to two dealth-signaling receptors, DR4 (CD261 / TRAIL-R1) and DR5 (CD262 / TRAIL-R2) .

• Certification:

  RUO

• Negative Species:

  Mouse

• Immunogen:

  Recombinant soluble fragment (aa 95-281) of human TRAIL.

• Target Antigen:

  CD253

• Clone:

  2E5

• Applications:

  FC (QC tested)

• Concentration:

  0.1 mg/mL

• Format:

  APC

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Hyer ML, Croxton R, Krajewska M, Krajewski S, Kress CL, Lu M, Suh N, Sporn MB, Cryns VL, Zapata JM, Reed JC: Synthetic triterpenoids cooperate with tumor necrosis factor-related apoptosis-inducing ligand to induce apoptosis of breast cancer cells. Cancer Res. 2005 Jun 1;65 (11) :4799-808., URL: https://www.ncbi.nlm.nih.gov/pubmed/15930300?dopt=, *Plasilova M, Zivny J, Jelinek J, Neuwirtova R, Cermak J, Necas E, Andera L, Stopka T: TRAIL (Apo2L) suppresses growth of primary human leukemia and myelodysplasia progenitors. Leukemia. 2002 Jan;16 (1) :67-73., URL: https://www.ncbi.nlm.nih.gov/pubmed/11840265?dopt=

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The antibody 2E5 reacts with an extracellular epitope within C-terminal half of TRAIL (APO-2L), a 21 kDa cytotoxic protein, activator of rapid apoptosis in tumor cells. TRAIL is mainly expressed in spleen, lung, prostate and also in many other tissues.

• Applications Notes:

  Flow cytometry: Recommended dilution: 1-5 μg/ml.

• Species:

  Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG1