Anti-Hu CD243 APC

CD243, also known as multidrug resistant protein 1 (MDR-1) or P-glycoprotein (Pgp) is an ATP binding cassette (ABC) -containing efflux transporter for xenobiotic lipophilic compounds with broad substrate specificity. It is responsible for decreased drug accumulation in multidrug-resistant cells and often mediates the development of resistance to anticancer drugs. This protein also functions as a transporter in the blood-brain barrier. It is expressed in many tissues, including the brain, liver, pancreas, testes, kidney, and blood (B, T, NK cells, but not monocytes) .

Human

NIH 3T3 cells transfected with human CD243 (MDR-1) cDNA

CD243

UIC2

FC (QC tested)

APC

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Kelley DJ, Pavelic ZP, Gapany M, Stambrook P, Pavelic L, Gapany S, Gluckman JL: Detection of P-glycoprotein in squamous cell carcinomas of the head and neck. Arch Otolaryngol Head Neck Surg. 1993 Apr;119 (4) :411-4., URL: http://www.ncbi.nlm.nih.gov/pubmed/8096141 , *Collnot EM, Baldes C, Schaefer UF, Edgar KJ, Wempe MF, Lehr CM: Vitamin E TPGS P-glycoprotein inhibition mechanism: influence on conformational flexibility, intracellular ATP levels, and role of time and site of access. Mol Pharm. 2010 Jun 7;7 (3) :642-51., URL: http://www.ncbi.nlm.nih.gov/pubmed/20205474 , *Goda K, Fenyvesi F, Bacsó Z, Nagy H, Márián T, Megyeri A, Krasznai Z, Juhász I, Vecsernyés M, Szabó G Jr: Complete inhibition of P-glycoprotein by simultaneous treatment with a distinct class of modulators and the UIC2 monoclonal antibody. J Pharmacol Exp Ther. 2007 Jan;320 (1) :81-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/17050779 , *Mechetner EB, Roninson IB: Efficient inhibition of P-glycoprotein-mediated multidrug resistance with a monoclonal antibody. Proc Natl Acad Sci U S A. 1992 Jul 1;89 (13) :5824-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/1352877 , *Chaudhary PM, Mechetner EB, Roninson IB: Expression and activity of the multidrug resistance P-glycoprotein in human peripheral blood lymphocytes. Blood. 1992 Dec 1;80 (11) :2735-9., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=chaudhary+pm+mechetner+eb

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The mouse monoclonal antibody UIC2 recognizes an extracellular epitope on CD243 (MDR-1), an approximately 170 kDa ABC transporter expressed on hematopoietic stem cells, B, T, and NK cells, or on many multidrug resistant cancer cells. This antibody preferentially recognizes CD243 in the process of transporting substrate.

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