Anti-BRG1 Purified

Brg1 (Brahma-related gene 1) is an ATPase subunit of SWI2/SNF2-like chromatin-remodeling complexes that enable access of regulatory and effector proteins in transcription, DNA repair and DNA replication. Although Brg1-containing complexes are not essential for general cell survival, they participate in transcriptional regulation of several hundred genes including those involved in interferon and stress response, immune cells differentiation, neurogenesis, cell cycle etc. and is absolutely necessary for mouse embryogenesis. Brg1 is also involved in cell growth arrest, senescence and tumour supression.

Human

Bacterially produced GST-fused protein representing 242 C-terminal amino acids of human Brg1.

BRG1

BRG-01

WB

1 mg/mL

Purified

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Ooi L, Belyaev ND, Miyake K, Wood IC, Buckley NJ: BRG1 chromatin remodeling activity is required for efficient chromatin binding by repressor element 1-silencing transcription factor (REST) and facilitates REST-mediated repression. J Biol Chem. 2006 Dec 22;281 (51) :38974-80. , URL: https://www.ncbi.nlm.nih.gov/pubmed/17023429?ordin, *Eroglu B, Wang G, Tu N, Sun X, Mivechi NF: Critical role of Brg1 member of the SWI/SNF chromatin remodeling complex during neurogenesis and neural crest induction in zebrafish. Dev Dyn. 2006 Oct;235 (10) :2722-35., URL: https://www.ncbi.nlm.nih.gov/pubmed/16894598?ordin, *Napolitano MA, Cipollaro M, Cascino A, Melone MA, Giordano A, Galderisi U: Brg1 chromatin remodeling factor is involved in cell growth arrest, apoptosis and senescence of rat mesenchymal stem cells. J Cell Sci. 2007 Aug 15;120 (Pt 16) :2904-11., URL: https://www.ncbi.nlm.nih.gov/pubmed/17666433?ordin, *Eroglu B, Wang G, Tu N, Sun X, Mivechi NF: Critical role of Brg1 member of the SWI/SNF chromatin remodeling complex during neurogenesis and neural crest induction in zebrafish. Dev Dyn. 2006 Oct;235 (10) :2722-35., URL: https://www.ncbi.nlm.nih.gov/pubmed/16894598?ordin, *Ooi L, Belyaev ND, Miyake K, Wood IC, Buckley NJ: BRG1 chromatin remodeling activity is required for efficient chromatin binding by repressor element 1-silencing transcription factor (REST) and facilitates REST-mediated repression. J Biol Chem. 2006 Dec 22;281 (51) :38974-80., URL: https://www.ncbi.nlm.nih.gov/pubmed/17023429?ordin

Store at 2-8°C. Do not freeze.

The antibody BRG-01 recognizes an epitope within C-terminal part of human Brg1, a 205 kDa catalytic subunit of SWI2/SNF2-family chromatin-remodeling complexes.

Western blotting: Recommended dilution: 10 μg/ml; positive control: HEK293 cells.