Anti-PKAc Purified

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Anti-PKAc Purified

CAT:

270-11-523-C100

Size:

0.1 mg

Price:

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Anti-PKAc Purified

Background: Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc proteinkinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit 8211 PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.
Specifications: The mouse monoclonal antibody 6D2.1 strongly reacts with human proteinkinase A (an intracellular antigen) catalytic (PKAc) alpha subunit, and weakly with PKAc gamma subunit (both around 40 kDa). The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog.
Certification: RUO
Host: Mouse
Species Reactivity: Human
Immunogen: Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A c alpha (PKAc alpha).
Target Antigen: PKAc
Isotype: IgG1
Clone: 6D2.1
Applications: Western blotting: Recommended dilution: 1 μg/mL positive control: HeLa human cervix carcinoma cell line. To detect PKAc gamma, use a more concentrated lysate from a tissue expressing this subunit (testis).
Validated Applications: WB
Concentration: 1 mg/mL
Format: Purified
Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Other References: *Doucas V, Shi Y, Miyamoto S, West A, Verma I, Evans RM: Cytoplasmic catalytic subunit of protein kinase A mediates cross-repression by NF-kappa B and the glucocorticoid receptor. Proc Natl Acad Sci U S A. 2000 Oct 2497(22):11893-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/11027313?ordin,*Ferraris JD, Persaud P, Williams CK, Chen Y, Burg MB: cAMP-independent role of PKA in tonicity-induced transactivation of tonicity-responsive enhancer/ osmotic response element-binding protein. Proc Natl Acad Sci U S A. 2002 Dec 2499(26):16800-5., URL: https://www.ncbi.nlm.nih.gov/pubmed/12482947?ordin,*Komagiri Y, Kitamura N: Comparison of effects of PKA catalytic subunit on I(h) and calcium channel currents in rat dorsal root ganglion cells. Biomed Res. 2007 Aug28(4):177-89., URL: https://www.ncbi.nlm.nih.gov/pubmed/17878598?ordin,*Kiger JA Jr, Eklund JL, Younger SH, O'Kane CJ: Transgenic inhibitors identify two roles for protein kinase A in Drosophila development. Genetics. 1999 May152(1):281-90., URL: https://www.ncbi.nlm.nih.gov/pubmed/10224260?ordin,*Beebe SJ, Oyen O, Sandberg M, Frøysa A, Hansson V, Jahnsen T: Molecular cloning of a tissue-specific protein kinase (C gamma) from human testis--representing a third isoform for the catalytic subunit of cAMP-dependent protein kinase. Mol Endocrinol. 1990 Mar4(3):465-75., URL: https://www.ncbi.nlm.nih.gov/pubmed/2342480?ordina,
Storage Conditions: Store at 2-8°C. Do not freeze.

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