Anti-DAXX Purified

Daxx is an apoptosis-modulating death domain-associated protein with functions in transcriptional regulation. Daxx functions both in cytoplasm, where it interacts with Fas, and in nucleus (residing in PML oncogenic domains), where it is involved in SUMO-dependent regulation of transcription and subnuclear compartmentalization. Daxx senzitizes the cells to apoptosis, but on the other hand, this protein may also serve in preventing apoptosis in the early embryo. Even regarding the transcription, Daxx can serve both as a corepressor and a coactivator. During ischemic stress, Daxx translocates from the nucleus to the cytoplasm, where in regulates sodium hydrogen exchanger isoform 1 (NHE1) .

Human

His6-tagged protein corresponding to amino acids 580-740 of human Daxx.

DAXX

DAXX-03

IP, WB, ICC

1 mg/mL

Purified

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Simova S, Klima M, Cermak L, Sourkova V, Andera L: Arf and Rho GAP adapter protein ARAP1 participates in the mobilization of TRAIL-R1/DR4 to the plasma membrane. Apoptosis. 2008 Mar;13 (3) :423-36., URL: https://www.ncbi.nlm.nih.gov/pubmed/18165900?ordin, *Svadlenka J, Brazina J, Hanzlikova H, Cermak L, Andera L: Multifunctional adaptor protein Daxx interacts with chromatin-remodelling ATPase Brg1. Biochem Biophys Rep. 2015 Dec 30;5:246-252., URL: https://www.ncbi.nlm.nih.gov/pubmed/28955830

*Jung YS, Kim HY, Kim J, Lee MG, Pouysségur J, Kim E: Physical Interactions and Functional Coupling between Daxx and Sodium Hydrogen Exchanger 1 in Ischemic Cell Death. J Biol Chem. 2008 Jan 11;283 (2) :1018-25., URL: https://www.ncbi.nlm.nih.gov/pubmed/18003619?ordin, *Shih HM, Chang CC, Kuo HY, Lin DY: Daxx mediates SUMO-dependent transcriptional control and subnuclear compartmentalization. Biochem Soc Trans. 2007 Dec;35 (Pt 6) :1397-400., URL: https://www.ncbi.nlm.nih.gov/pubmed/18031230?ordin, *Li Q, Wang X, Wu X, Rui Y, Liu W, Wang J, Wang X, Liou YC, Ye Z, Lin SC: Daxx cooperates with the Axin/HIPK2/p53 complex to induce cell death. Cancer Res. 2007 Jan 1;67 (1) :66-74., URL: https://www.ncbi.nlm.nih.gov/pubmed/17210684?ordin, *Croxton R, Puto LA, de Belle I, Thomas M, Torii S, Hanaii F, Cuddy M, Reed JC. Daxx represses expression of a subset of antiapoptotic genes regulated by nuclear factor-kappaB. Cancer Res. 2006 Sep 15;66 (18) :9026-35., URL: https://www.ncbi.nlm.nih.gov/pubmed/16982744?ordin, *Muromoto R, Ishida M, Sugiyama K, Sekine Y, Oritani K, Shimoda K, Matsuda T: Sumoylation of Daxx regulates IFN-induced growth suppression of B lymphocytes and the hormone receptor-mediated transactivation. J Immunol. 2006 Jul 15;177 (2) :1160-70., URL: https://www.ncbi.nlm.nih.gov/pubmed/16818774?ordin

Store at 2-8°C. Do not freeze.

The mouse monoclonal antibody DAXX-03 reacts with C-terminal part of Daxx, a 110 kDa intracellular protein.

Western blotting: Recommended dilution: 2 μg/ml.