Anti-Clusterin Purified

Clusterin (APO J, SGP-2, TRPM-2, SP-40, pADHC-9, CLJ, T64, GP III, XIP8) is a 75-80 kD disulfide-linked heterodimeric protein containing about 30% of N-linked carbohydrate rich in sialic acid but truncated forms targeted to the nucleus have also been identified. It is a conserved secreted glycoprotein expressed by a wide range of tissues and being implicated in many physiological processes, including e.g. lipid transportation, complement inhibition, tissue remodeling, membrane recycling, or clearence of cellular debris. It is nearly ubiqitously expressed in most mammalian tissues and can be found in plasma, milk, urine, cerebrospinal fluid and semen. Clusterin is able to bind and form complexes with numerous partners (immunoglobulins, lipids, heparin, bacteria, complement components, paraoxonase, beta amyloid, leptin etc.) and is expressed in many pathological and clinically relevant situations including cancer, organ regeneration, infection, Alzheimer disease, retinitis pigmentosa, myocardial infarction, renal tubular damage, autoimmunity and others. A genuine function of clusterin is still enigmatic.

Human

Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Clusterin

Hs-3

WB, IHC-P, ICC, ELISA

1 mg/mL

Purified

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Capkova J, Geussova G, Peknicova J: New monoclonal antibody to human apolipoprotein J. Folia Biol (Praha) . 2002;48 (1) :40-2., URL: , *Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov;15 (11) :909-912., URL: https://www.ncbi.nlm.nih.gov/pubmed/30377371

*Shannan B, Seifert M, Boothman DA, Tilgen W, Reichrath J: Clusterin and DNA repair: a new function in cancer for a key player in apoptosis and cell cycle control. J Mol Histol. 2006 Sep;37 (5-7) :183-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/17048076?ordin, *Rodríguez-Piñeiro AM, de la Cadena MP, López-Saco A, Rodríguez-Berrocal FJ: Differential expression of serum clusterin isoforms in colorectal cancer. Mol Cell Proteomics. 2006 Sep;5 (9) :1647-57., URL: https://www.ncbi.nlm.nih.gov/pubmed/16854844?ordin, *Pajak B, Orzechowski A: Clusterin: the missing link in the calcium-dependent resistance of cancer cells to apoptogenic stimuli. Postepy Hig Med Dosw (Online) . 2006;60:45-51., URL: https://www.ncbi.nlm.nih.gov/pubmed/16474275?ordin, *Cochrane DR, Wang Z, Muramaki M, Gleave ME, Nelson CC: Differential regulation of clusterin and its isoforms by androgens in prostate cells. J Biol Chem. 2007 Jan 26;282 (4) :2278-87., URL: https://www.ncbi.nlm.nih.gov/pubmed/17148459?ordin, *Schepeler T, Mansilla F, Christensen LL, Orntoft TF, Andersen CL: Clusterin expression can be modulated by changes in TCF1-mediated Wnt signaling. J Mol Signal. 2007 Jul 16;2:6., URL: https://www.ncbi.nlm.nih.gov/pubmed/17634137?ordin

Store at 2-8°C. Do not freeze.

The mouse monoclonal antibody Hs-3 recognizes human clusterin (apolipoprotein J), a conserved secreted glycoprotein.

Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml; positive tissue: Sertoli cells. Western blotting: Recommended dilution: 1 μg/ml; positive material: seminal plasma.