Anti-HLA-E Purified

HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells) .

Non-human primates, Human

Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.

HLA-E

MEM-E/06

FC (QC tested), IP, IHC-P

1 mg/mL

Purified

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Lo Monaco E, Tremante E, Cerboni C, Melucci E, Sibilio L, Zingoni A, Nicotra MR, Natali PG, Giacomini P: Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells. Neoplasia. 2011 Sep;13 (9) :822-30., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=tremante+e+cerboni+c , *Dambaeva SV, Bondarenko GI, Grendell RL, Kravitz RH, Durning M, Golos TG: Non-classical MHC-E (Mamu-E) expression in the rhesus monkey placenta. Placenta. 2008 Jan;29 (1) :58-70., URL: http://www.ncbi.nlm.nih.gov/pubmed/17996936 , *Gonen-Gross T, Achdout H, Arnon TI, Gazit R, Stern N, Horejsí V, Goldman-Wohl D, Yagel S, Mandelboim O: The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules. J Immunol. 2005 Oct 15;175 (8) :4866-74., URL: https://www.ncbi.nlm.nih.gov/pubmed/16210588?ordin, *Palmisano GL, Contardi E, Morabito A, Gargaglione V, Ferrara GB, Pistillo MP.: HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines. Hum Immunol. 2005 Jan;66 (1) :1-12., URL: https://www.ncbi.nlm.nih.gov/pubmed/15620456?dopt=, *Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N.: Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 Mar;64 (3) :315-26., URL: https://www.ncbi.nlm.nih.gov/pubmed/12590976?dopt=

Store at 2-8°C. Do not freeze.

The antibody MEM-E/06 recognizes an extracellular epitope on native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on many types of the human cells. The published results showed that the antibody cross-reacts with some classical MHC Class I molecules (HLA-A3, -A11, -B7) . However, the recent Workshop I Session on the 3rd International Conference on HLA-G (Paris, July 2003) confirmed that the antibody exhibits much broader cross-reactivity classical MHC Class I antigens, namely with HLA-A24, -A32, -B8, -B15, -B27, -B35, -B44, -B54, -C3, -C4, -C5, -C7.

Immunohistochemistry (paraffin sections) : Recommended dilution: 10 μg/ml; positive tissue: spleen. Flow cytometry: Recommended dilution: 1-4 µg/ml