Anti-HLA-E Purified

Anti-HLA-E Purified

• Background: HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells).
• Specifications: The antibody MEM-E/02 specifically reacts with an extracellular epitope on denaturated heavy chain of human HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib nonclassical) and it is expressed on the surface of all human cell types.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human, Non-human primates
• Immunogen: Recombinant human HLA-E denaturated heavy chain.
• Target Antigen: HLA-E
• Isotype: IgG1
• Clone: MEM-E/02
• Applications: Immunohistochemistry (paraffin sections): Recommended dilution: 5-10 μg/mL positive control: human placenta heat antigen retrieval (sodium citrate), incubation with mAb: 1 hour / RT, detection DAB.
• Validated Applications: WB, IHC-P
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Spaans VM, Peters AA, Fleuren GJ, Jordanova ES: HLA-E expression in cervical adenocarcinomas: association with improved long-term survival. J Transl Med. 2012 Sep 410:184., URL: https://www.ncbi.nlm.nih.gov/pubmed/22947189 ,*Lo Monaco E, Tremante E, Cerboni C, Melucci E, Sibilio L, Zingoni A, Nicotra MR, Natali PG, Giacomini P: Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells. Neoplasia. 2011 Sep13(9):822-30., URL: http://www.ncbi.nlm.nih.gov/pubmed/?term=tremante+e+cerboni+c ,*Menier C, Saez B, Horejsi V, Martinozzi S, Krawice-Radanne I, Bruel S, Le Danff C, Reboul M, Hilgert I, Rabreau M, Larrad ML, Pla M, Carosella ED, Rouas-Freiss N: Characterization of monoclonal antibodies recognizing HLA-G or HLA-E: new tools to analyze the expression of nonclassical HLA class I molecules. Hum Immunol. 2003 Mar64(3):315-26. , URL: https://www.ncbi.nlm.nih.gov/pubmed/12590976?dopt=,*Dambaeva SV, Bondarenko GI, Grendell RL, Kravitz RH, Durning M, Golos TG: Non-classical MHC-E (Mamu-E) expression in the rhesus monkey placenta. Placenta. 2008 Jan29(1):58-70., URL: http://www.ncbi.nlm.nih.gov/pubmed/17996936 ,
• Storage Conditions: Store at 2-8°C. Do not freeze.