Anti-Ms MHC Class II Purified Azide Free

• Description:

  MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.

• Certification:

  RUO

• Immunogen:

  Activated C57BL/6 mouse spleen cells

• Target Antigen:

  MHC Class II

• Clone:

  M5/114

• Applications:

  FC (QC tested), IP, WB, IHC-P, IHC-F, FUNC

• Concentration:

  1 mg/mL

• Format:

  Purified Azide Free

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4

• References & Citations:

  *Clausen BE, Waldburger JM, Schwenk F, Barras E, Mach B, Rajewsky K, Förster I, Reith W: Residual MHC class II expression on mature dendritic cells and activated B cells in RFX5-deficient mice. Immunity. 1998 Feb;8 (2) :143-55. , URL: https://www.ncbi.nlm.nih.gov/pubmed/9491996?itool=, *Zang W, Kalache S, Lin M, Schroppel B, Murphy B: MHC Class II-mediated apoptosis by a nonpolymorphic MHC Class II peptide proceeds by activation of protein kinase C. J Am Soc Nephrol. 2005 Dec;16 (12) :3661-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/16221866?itool, *Kuwano Y, Pražma CM, Yazawa N, Watanabe R, Ishiura N, Kumanogoh A, Okochi H, Tamaki K, Fujimoto M, Tedder TF: CD83 influences cell-surface MHC class II expression on B cells and other antigen-presenting cells. Int Immunol. 2007 Aug;19 (8) :977-92., URL: https://www.ncbi.nlm.nih.gov/pubmed/17804692?itool, *Beers C, Burich A, Kleijmeer MJ, Griffith JM, Wong P, Rudensky AY: Cathepsin S controls MHC class II-mediated antigen presentation by epithelial cells in vivo. J Immunol. 2005 Feb 1;174 (3) :1205-12., URL: https://www.ncbi.nlm.nih.gov/pubmed/15661874?itool, *Kleijmeer M, Ramm G, Schuurhuis D, Griffith J, Rescigno M, Ricciardi-Castagnoli P, Rudensky AY, Ossendorp F, Melief CJ, Stoorvogel W, Geuze HJ: Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells. J Cell Biol. 2001 Oct 1;155 (1) :53-63., URL: https://www.ncbi.nlm.nih.gov/pubmed/11581285?itool, *De Souza Leao S, Lang T, Prina E, Hellio R, Antoine JC: Intracellular Leishmania amazonensis amastigotes internalize and degrade MHC class II molecules of their host cells. J Cell Sci. 1995 Oct;108 (Pt 10) :3219-31., URL: https://www.ncbi.nlm.nih.gov/pubmed/7593283?itool=, *Viville S, Neefjes J, Lotteau V, Dierich A, Lemeur M, Ploegh H, Benoist C, Mathis D: Mice lacking the MHC class II-associated invariant chain. Cell. 1993 Feb 26;72 (4) :635-48., URL: https://www.ncbi.nlm.nih.gov/pubmed/7679955?itool=, *Bhattacharya A, Dorf ME, Springer TA: A shared alloantigenic determinant on Ia antigens encoded by the I-A and I-E subregions: evidence for I region gene duplication. J Immunol. 1981 Dec;127 (6) :2488-95., URL: https://www.ncbi.nlm.nih.gov/pubmed/6170707?itool=

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Specificity:

  The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.

• Species:

  Mouse

• CAS Number:

  9007-83-4

• Isotype:

  Rat IgG2b