Anti-Ms CD11a Purified

Anti-Ms CD11a Purified

• Background: CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.
• Specifications: The rat monoclonal antibody M17/4 reacts with an extracellular epitope of CD11a (alpha-subunit of murine LFA-1), a 180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Rat
• Species Reactivity: Mouse
• Immunogen: C57BL/6 mouse splenic secondary cytotoxic T lymphocytes
• Target Antigen: CD11a
• Isotype: IgG2a
• Clone: M17/4
• Applications: Flow cytometry: Recommended dilution: 1 μg/mL. Immunohistochemistry (frozen sections): Positive tissue: murine spleen or thymus, acetone fixation.
• Validated Applications: FC, IP, IHC-F
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
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• Storage Conditions: Store at 2-8°C. Do not freeze.