Anti-Ms Blnk Purified

Anti-Ms Blnk Purified

• Background: SLP65 / BLNK (SH2 domain-containing leukocyte-specific phosphoprotein of 65 kDa B cell linker protein), also known as BASH, is an adaptor protein that plays key role in B cell activation initiated by cross-linking the B cell receptor (BCR). Phosphorylated by Syk tyrosine kinase, SLP65 serves as a scaffold for Btk tyrosine kinase, Vav1 guanine nucleotide exchange factor, phospholipase C gamma2, as well as Grb2 and Nck adaptor proteins thus represents a central linker protein that bridges the BCR-associated kinases with a multitude of signaling pathways.
• Specifications: The Polyclonal antibody reacts with mouse SLP65 / BLNK, a cytosolic adaptor protein identified as two phosphoproteins migrating at 68 and 70 kDa in SDS/PAGE (alternatively spliced forms of human SLP65).
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Rabbit
• Species Reactivity: Mouse
• Immunogen: A fusion protein representing amino acids 171-356 of human BLNK.
• Target Antigen: Blnk
• Clonality: Polyclonal
• Clone: PAb (411)
• Applications: Western blotting: Reducing conditions.
• Validated Applications: WB
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Other References: *Fu C, Turck CW, Kurosaki T, Chan AC: BLNK: a central linker protein in B cell activation. Immunity. 1998 Jul9(1):93-103., URL: https://www.ncbi.nlm.nih.gov/pubmed/9697839?ordina,*Jumaa H, Mitterer M, Reth M, Nielsen PJ: The absence of SLP65 and Btk blocks B cell development at the preB cell receptor-positive stage. Eur J Immunol. 2001 Jul31(7):2164-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/11449370?ordin,*Kersseboom R, Middendorp S, Dingjan GM, Dahlenborg K, Reth M, Jumaa H, Hendriks RW: Bruton's tyrosine kinase cooperates with the B cell linker protein SLP-65 as a tumor suppressor in Pre-B cells. J Exp Med. 2003 Jul 7198(1):91-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/12835482?ordin,*Kersseboom R, Ta VB, Zijlstra AJ, Middendorp S, Jumaa H, van Loo PF, Hendriks RW: Bruton's tyrosine kinase and SLP-65 regulate pre-B cell differentiation and the induction of Ig light chain gene rearrangement. J Immunol. 2006 Apr 15176(8):4543-52., URL: https://www.ncbi.nlm.nih.gov/pubmed/16585544?ordin,*Koretzky GA, Abtahian F, Silverman MA: SLP76 and SLP65: complex regulation of signalling in lymphocytes and beyond. Nat Rev Immunol. 2006 Jan6(1):67-78., URL: https://www.ncbi.nlm.nih.gov/pubmed/16493428?ordin,
• Storage Conditions: Store at 2-8°C. Do not freeze.