Anti-Hu CD34 Purified Azide Free

Anti-Hu CD34 Purified Azide Free

• Background: CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
• Specifications: The antibody QBEnd-10 reacts with an extracellular class II epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells it is gradually lost on progenitor cells. This antibody has been also used as an endothelial marker.
• Certification: RUO
• Host: Mouse
• Species Reactivity: Non-human primates, Human
• Negative Species: Dog, Sheep, Rat, Cow
• Immunogen: Human endothelial vesicles
• Target Antigen: CD34
• Isotype: IgG1
• Clone: QBEnd-10
• Applications: Functional application: The antibody QBEnd-10 induces homotypic adhesion of leukemic cell line. Flow cytometry: Recommended dilution: 5 μg/mL. Immunohistochemistry (paraffin sections): Recommended dilution: 2-8 μg/mL.Western blotting: Recommended dilution: 1-2 μg/mL, positive control: TF-1 cells.
• Validated Applications: FC, IP, WB, IHC-P, IHC-F, Functional assays
• Concentration: 1 mg/mL
• Format: Purified Azide Free
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• References & Citations: *Schlingemann RO, Rietveld FJ, de Waal RM, Bradley NJ, Skene AI, Davies AJ, Greaves MF, Denekamp J, Ruiter DJ: Leukocyte antigen CD34 is expressed by a subset of cultured endothelial cells and on endothelial abluminal microprocesses in the tumor stroma. Lab Invest. 1990 Jun62(6):690-6., URL: https://www.ncbi.nlm.nih.gov/pubmed/1694254?ordina,*Ramani P, Bradley NJ, Fletcher CD: QBEND/10, a new monoclonal antibody to endothelium: assessment of its diagnostic utility in paraffin sections. Histopathology. 1990 Sep17(3):237-42., URL: https://www.ncbi.nlm.nih.gov/pubmed/2242851?ordina,*Kuzu I, Bicknell R, Harris AL, Jones M, Gatter KC, Mason DY: Heterogeneity of vascular endothelial cells with relevance to diagnosis of vascular tumours. J Clin Pathol. 1992 Feb45(2):143-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/1371777?ordina,*Sutherland DR, Marsh JC, Davidson J, Baker MA, Keating A, Mellors A: Differential sensitivity of CD34 epitopes to cleavage by Pasteurella haemolytica glycoprotease: implications for purification of CD34-positive progenitor cells. Exp Hematol. 1992 Jun20(5):590-9., URL: https://www.ncbi.nlm.nih.gov/pubmed/1375160?ordina,*Grimsley PG, Amos TA, Gordon MY, Greaves MF: Rapid positive selection of CD34+ cells using magnetic microspheres coated with monoclonal antibody QBEND/10 linked via a cleavable disulphide bond. Leukemia. 1993 Jun7(6):898-908., URL: https://www.ncbi.nlm.nih.gov/pubmed/7684800?ordina,*Poblet E, Jimenez-Acosta F, Rocamora A: QBEND/10 (anti-CD34 antibody) in external root sheath cells and follicular tumors. J Cutan Pathol. 1994 Jun21(3):224-8., URL: https://www.ncbi.nlm.nih.gov/pubmed/7525669?ordina,*Traoré Y, Hirn J: Certain anti-CD34 monoclonal antibodies induce homotypic adhesion of leukemic cell lines in a CD18-dependent and a CD18-independent way. Eur J Immunol. 1994 Oct24(10):2304-11., URL: https://www.ncbi.nlm.nih.gov/pubmed/7523134?ordina,*Koumarianou A, Economopoulou P, Katsaounis P, Laschos K, Arapantoni-Dadioti P, Martikos G, Rogdakis A, Tzanakis N, Boukovinas I: Gastrointestinal stromal tumors (GIST): A prospective analysis and an update on biomarkers and current treatment concepts. Biomark Cancer 2015 May 217(Suppl 1):1-7. , URL: https://pubmed.ncbi.nlm.nih.gov/26056505/,*Castrechini NM, Murthi P, Gude NM, Erwich JJ, Gronthos S, Zannettino A, Brennecke SP, Kalionis B: Placenta. Mesenchymal stem cells in human placental chorionic villi reside in a vascular niche. 2010 Mar31(3):203-12. , URL: https://pubmed.ncbi.nlm.nih.gov/20060164/,*Bonelli P, Nicolussi P, Manetti R, Antuofermo E, Dattena M: Monoclonal antigen do not cross-react with ovine umbilical cord blood cells. Ital J Anim Sci 2010, vol.9:e6., URL: https://www.tandfonline.com/doi/full/10.4081/ijas.2010.e6,
• Other References: *Krauter J, Hartl M, Hambach L, Kohlenberg A, Gunsilius E, Ganser A, Heil G: Receptor-mediated endocytosis of CD34 on hematopoietic cells after stimulation with the monoclonal antibody anti-HPCA-1. J Hematother Stem Cell Res. 2001 Dec10(6):863-71., URL: http://www.ncbi.nlm.nih.gov/pubmed/11798512?ordina,*Dao MA, Arevalo J, Nolta JA: Reversibility of CD34 expression on human hematopoietic stem cells that retain the capacity for secondary reconstitution. Blood. 2003 Jan 1101(1):112-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/12393633?ordina,*Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Gulati S, Luthra PM: Three-dimensional structure prediction of the interaction of CD34 with the SH3 domain of Crk-L. Stem Cells Dev. 2005 Oct14(5):470-7., URL: http://www.ncbi.nlm.nih.gov/pubmed/16305332?ordina,*Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Luthra PM: Hematopoietic stem cell antigen CD34: role in adhesion or homing. Stem Cells Dev. 2006 Jun15(3):305-13., URL: http://www.ncbi.nlm.nih.gov/pubmed/16846369?ordina,
• Storage Conditions: Store at 2-8°C. Do not freeze.