Anti-Hu CD34 Purified

Anti-Hu CD34 Purified

• Background: CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
• Specifications: The mouse monoclonal antibody 4H11[APG] reacts with extracellular class III epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells it is gradually lost on progenitor cells. The antibody 4H11[APG] completely blocks binding of class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.
• Target Antigen: CD34
• Isotype: IgG1
• Clone: 4H11[APG]
• Applications: Western blotting: Recommended dilution: 1-2 μg/mL positive control: Kg-1a, TF-1 cells, non-reducing conditions. Flow cytometry: Recommended dilution: 2 μg/mL. Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL positive tissue: placenta endothelium.
• Validated Applications: FC, WB, IHC-P, ICC
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Elknerová K, Lacinová Z, Soucek J, Marinov I, Stöckbauer P: Growth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines. Neoplasma. 200754(4):311-20., URL: http://www.ncbi.nlm.nih.gov/pubmed/17822321?itool=,
• Other References: *Krauter J, Hartl M, Hambach L, Kohlenberg A, Gunsilius E, Ganser A, Heil G: Receptor-mediated endocytosis of CD34 on hematopoietic cells after stimulation with the monoclonal antibody anti-HPCA-1. J Hematother Stem Cell Res. 2001 Dec10(6):863-71., URL: http://www.ncbi.nlm.nih.gov/pubmed/11798512?ordina,*Dao MA, Arevalo J, Nolta JA: Reversibility of CD34 expression on human hematopoietic stem cells that retain the capacity for secondary reconstitution. Blood. 2003 Jan 1101(1):112-8., URL: http://www.ncbi.nlm.nih.gov/pubmed/12393633?ordina,*Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Gulati S, Luthra PM: Three-dimensional structure prediction of the interaction of CD34 with the SH3 domain of Crk-L. Stem Cells Dev. 2005 Oct14(5):470-7., URL: http://www.ncbi.nlm.nih.gov/pubmed/16305332?ordina,*Gangenahalli GU, Singh VK, Verma YK, Gupta P, Sharma RK, Chandra R, Luthra PM: Hematopoietic stem cell antigen CD34: role in adhesion or homing. Stem Cells Dev. 2006 Jun15(3):305-13., URL: http://www.ncbi.nlm.nih.gov/pubmed/16846369?ordina,
• Storage Conditions: Store at 2-8°C. Do not freeze.