Mouse anti Human CD14, conjugated with FITC

• Background:

  Identification of Human Monocyte/Macrophage cells expressing the 55 kDa M.W. surface antigen. CD14 is present on 70-93% of Monocyte/Macrophage cells in normal peripheral blood.

• Synonyms:

  CD14 FITC

• UniProt:

  P08571

• Host:

  Mouse

• Species Reactivity:

  Human

• Isotype:

  IgG2b

• Clone:

  FWKW

• Conjugation:

  FITC

• Type:

  Single-Color Reagent

• Applications:

  Flow Cytometry

• Field of Research:

  Flow Cytometry

• Purification Method:

  Protein A/G Chromatography

• Assay Principle:

  PBMC: Add10 µL of MAB/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2 to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature (20ºC). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjµgates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.

• Stability:

  See expiration date on vial

• Concentration:

  Titered for flow cytometry

• Form:

  Provided as solution in phosphate buffered saline with 0,08% sodium azide and 0,2% carrier protein

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. Immunocytochemical investigation of normal and chronic lymphocytic leukaemia lymphocytes reveals unexpectedly frequent reactivity with some myelomonocytic associated antibodies. Woessner S; Florensa L; Besses C; Vila RM; Galles C; Palou L; Sabrafen JS Leuk Res 1992;16(5):505-10 2. Effect of cytokines and lipopolysaccharide on CD14 antigen expression in Human monocytes and macrophages. Landmann R; Ludwig C; Obrist R; Obrecht JP J Cell Biochem 1991 Dec;47(4):317-29 _x000D_ 3. Surface markers in adult acute myeloblastic leukemia: correlation of CD19+, CD34+ and CD14+/DR--phenotypes with shorter survival. Groupe d'Etude Immunologique des Leucemies (GEIL). Solary E; Casasnovas RO; Campos L; Bene MC; Faure G; Maingon P; Falkenrodt A. Leukemia 1992 May;6(5):393-9 _x000D_ 4. In situ hybridization of interleukin-1 in CD14-positive cells in rheumatoid arthritis. Wood NC; Dickens E; Symons JA; Duff GW Clin Immunol Immunopathol 1992 Mar;62(3):295-300_x000D_ 5. Expression of CD54, CD58, CD14, and HLA-DR on macrophages and macrophage-derived accessory cells and their accessory capacity. Ocklind G; Friedrichs D; Peters JH Immunol Lett 1992 Feb 15;31(3):253-8 _x000D_ 6. IL-4 decreases the expression of the monocyte differentiation marker CD14, paralleled by an increasing accessory potency. Ruppert J; Friedrichs D; Xu H; Peters JH Immunobiology 1991 Aug;182(5):449-64

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  Product should be stored at 4-8ºC; DO NOT FREEZE

• Functional Analysis:

  Flow Cytometry Staining

• CAS Number:

  9007-83-4