Mouse anti Human CD14, conjugated with FITC

Mouse anti Human CD14, conjugated with FITC

• Background: Identification of Human Monocyte/Macrophage cells expressing the 55 kDa M.W. surface antigen. CD14 is present on 70-93% of Monocyte/Macrophage cells in normal peripheral blood.
• Synonyms: CD14 FITC
• CAS Number: 9007-83-4
• UniProt: P08571
• Host: Mouse
• Species Reactivity: Human
• Isotype: IgG2b
• Clone: FWKW
• Conjugation: FITC
• Type: Single-Color Reagent
• Applications: Flow Cytometry
• Field of Research: Flow Cytometry
• Purification Method: Protein A/G Chromatography
• Assay Principle: PBMC: Add10 µL of MAB/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2 to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature (20ºC). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjµgates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.
• Stability: See expiration date on vial
• Concentration: Titered for flow cytometry
• Form: Provided as solution in phosphate buffered saline with 0,08% sodium azide and 0,2% carrier protein
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• References & Citations: 1. Immunocytochemical investigation of normal and chronic lymphocytic leukaemia lymphocytes reveals unexpectedly frequent reactivity with some myelomonocytic associated antibodies. Woessner S; Florensa L; Besses C; Vila RM; Galles C; Palou L; Sabrafen JS Leuk Res 1992;16(5):505-10 2. Effect of cytokines and lipopolysaccharide on CD14 antigen expression in Human monocytes and macrophages. Landmann R; Ludwig C; Obrist R; Obrecht JP J Cell Biochem 1991 Dec;47(4):317-29 _x000D_ 3. Surface markers in adult acute myeloblastic leukemia: correlation of CD19+, CD34+ and CD14+/DR--phenotypes with shorter survival. Groupe d'Etude Immunologique des Leucemies (GEIL). Solary E; Casasnovas RO; Campos L; Bene MC; Faure G; Maingon P; Falkenrodt A. Leukemia 1992 May;6(5):393-9 _x000D_ 4. In situ hybridization of interleukin-1 in CD14-positive cells in rheumatoid arthritis. Wood NC; Dickens E; Symons JA; Duff GW Clin Immunol Immunopathol 1992 Mar;62(3):295-300_x000D_ 5. Expression of CD54, CD58, CD14, and HLA-DR on macrophages and macrophage-derived accessory cells and their accessory capacity. Ocklind G; Friedrichs D; Peters JH Immunol Lett 1992 Feb 15;31(3):253-8 _x000D_ 6. IL-4 decreases the expression of the monocyte differentiation marker CD14, paralleled by an increasing accessory potency. Ruppert J; Friedrichs D; Xu H; Peters JH Immunobiology 1991 Aug;182(5):449-64
• Shipping Conditions: Ambient Temperature
• Storage Conditions: Product should be stored at 4-8ºC; DO NOT FREEZE
• Functional Analysis: Flow Cytometry Staining