Anti-Hu CD369 APC

• Description:

  CD369 (dectin-1, beta-glucan receptor) is a 33 kDa type II transmembrane glycoprotein of lectin family, and serves as a part of innate immunity system by binding to beta-glucan polymers, which are typical for yeast and mycobacterial cell walls. CD369 is expressed predominantly on dendritic cells, but it can be detected also on monocytes, macrophages, mast cells, eosinophils, B cells, endothelial cells, and sometimes also on some T cell subsets.

• Certification:

  RUO

• Immunogen:

  Extracellular part of human CD369 with hIgG FC tag

• Target Antigen:

  CD369

• Clone:

  15E2

• Applications:

  FC (QC tested)

• Format:

  APC

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Ni L, Gayet I, Zurawski S, Duluc D, Flamar AL, Li XH, O'Bar A, Clayton S, Palucka AK, G Zurawski, Banchereau J, Oh SK: Concomitant activation and antigen uptake via human dectin-1 results in potent antigen-specific CD8+ T cell responses. J Immunol. 2010 Sep 15;185 (6) :3504-13, URL: https://pubmed.ncbi.nlm.nih.gov/20729328/?from_term=ni+l+gayet+i+zurawski+s&from_pos=2, *Fischer M, Müller JP, Spies-Weisshart B, Gräfe C, Kurzai O, Hünniger K, Hochhaus A, Scholl S, Schnetzke U. Isoform localization of dectin-1 regulates the signaling quality of anti-fungal immunity. Eur J Immunol. 2017 May;47 (5) :848-859 (FC), URL: https://pubmed.ncbi.nlm.nih.gov/28303575/?from_term=scholl+s+hochhaus+a&from_pos=5, *Joo H, Upchurch K, Zhang W, Ni L, Li D, Xue Y, Li XH, Hori T, Zurawski S, Liu YJ, Zurawski G, Oh S: Opposing roles of dectin-1 expressed on human plasmacytoid dendritic cells and myeloid dendritic cells in Th2 polarization. J Immunol. 2015 Aug 15;195 (4) :1723-31, URL: https://pubmed.ncbi.nlm.nih.gov/26123355/?from_term=zhang+w+xue+y&from_page=6&from_pos=10

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody 15E2 recognizes an extracellular epitope LWEDGSTFSSN of human CD369 (Dectin-1), a 33 kDa transmembrane glycoprotein expressed predominantly on dendritic cells.

• Applications Notes:

  6

• Species:

  Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG2a kappa