Anti-Hu SUSD2

SUSD2 (sushi domain containing protein 2) is a type I transmembrane protein, that serves as an important marker of bone marrow-derived mesenchymal stem-like cells (bone marrow stromal cells) . These pluripotent cells are important for techniques of autologous cell therapy, and can be collected from e.g. endometrium, or palatine tonsil. SUSD2 seems to be a tumor supresor, and is down-regulated in colon cancer tissues, whereas it is highly expressed e.g. in breast cancer.

Human

WERI-RB-1 retinoblastoma cells

SUSD2

W5C5

ICC, FC (QC tested)

1 mg/mL

Purified

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

*Bredenkamp N, Stirparo GG, Nichols J, Smith A, Guo G: The cell-surface marker sushi containing domain 2 facilitates establishment of human naive pluripotent stem cells. Stem Cell Reports. 2019 Jun 11;12 (6) :1212-1222., URL: https://pubmed.ncbi.nlm.nih.gov/31031191/, *Murakami K, Lee YH, Lucas ES, Chan YW, Durairaj RP, Takeda S, Moore JD, Tan BK, Quenby S, Chan JK, Gargett CE, Brosens JJ: Decidualization induces a secretome switch in perivascular niche cells of the human endometrium. Endocrinology. 2014 Nov;155 (11) :4542-53., URL: https://www.ncbi.nlm.nih.gov/pubmed/25116707, *Lee BJ, Kang DW, Park HY, Song JS, Kim JM, Jang JY, Lee JC, Wang SG, Jung JS, Shin SC: Isolation and Localization of Mesenchymal Stem Cells in Human Palatine Tonsil by W5C5 (SUSD2) . Cell Physiol Biochem. 2016;38 (1) :83-93., URL: https://www.ncbi.nlm.nih.gov/pubmed/26741828, *Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W: Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007 Jun;1106:262-71. , URL: https://www.ncbi.nlm.nih.gov/pubmed/17395729, *Sivasubramaniyan K, Harichandan A, Schumann S, Sobiesiak M, Lengerke C, Maurer A, Kalbacher H, Bühring HJ: Prospective isolation of mesenchymal stem cells from human bone marrow using novel antibodies directed against Sushi domain containing 2. Stem Cells Dev. 2013 Jul 1;22 (13) :1944-54., URL: https://www.ncbi.nlm.nih.gov/pubmed/23406305, *Ulrich D, Tan KS, Deane J, Schwab K, Cheong A, Rosamilia A, Gargett CE: Mesenchymal stem/stromal cells in post-menopausal endometrium. Hum Reprod. 2014 Sep;29 (9) :1895-905., URL: https://www.ncbi.nlm.nih.gov/pubmed/24964924

Store at 2-8°C. Do not freeze.

The mouse monoclonal antibody W5C5 recognizes an extracellular epitope of SUSD2, a type I transmembrane protein expressed on mesenchymal stem-like cells. This antibody selectively binds to a MSCs in both bone marrow and endometrium or tonsil, and can be used for their identification and isolation.

Flow cytometry: Recommended dilution: 1-4 µg/ml