Anti-Hu CD36 APC

Anti-Hu CD36 APC

• Background: CD36 (fatty acid translocase, FAT) is an 88 kDa ditopic glycosylated protein that belongs to the class B family of scavenger receptors. CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and it is rapidly induced on follicular B cells in vitro upon TLR and CD40 stimulation. CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. Similarly to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids.
• Specifications: The antibody TR9 reacts with an extracellular epitope of CD36 (GPIIIb), a 85 kDa integral membrane glycoprotein expressed on platelets, macrophages, endothelial cells, early erythroid cells and megakaryocytes. The antibody TR9 cross-blocks binding of FITC-labeled standard antibody OKM5. Anti-CD36 antibodies inhibit adhesive functions (e.g. adherence of infected erythrocytes to target cells).
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Platelets
• Target Antigen: CD36
• Isotype: IgG1
• Clone: TR9
• Applications: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 mL) is sufficient for 100 tests.
• Validated Applications: FC
• Format: APC
• Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Zamora C, Cantó E, Nieto JC, Ortiz MA, Diaz-Torné C, Diaz-Lopez C, Llobet JM, Juarez C, Vidal S: Functional consequences of platelet binding to T lymphocytes in inflammation. J Leukoc Biol. 2013 Sep94(3):521-9. , URL: https://www.ncbi.nlm.nih.gov/pubmed/23801652,*Bertrand G, Renac V, Lefaix MC, Nivet C, Trudel E, Richard L: Neonatal Intracranial Hemorrhage with a Dramatic Outcome Due to Maternal Anti CD36 Antibodies. Reports 2019, 2(1), 7 doi:10.3390/reports2010007 , URL: https://www.mdpi.com/2571-841X/2/1/7,*Berre S, Gaudin R, Cunha de Alencar B, Desdouits M, Chabaud M, Naffakh N, Rabaza-Gairi M, Gobert FX, Jouve M, Benaroch P: CD36-specific antibodies block release of HIV-1 from infected primary macrophages and its transmission to T cells. J Exp Med. 2013 Nov 18210(12):2523-38., URL: https://www.ncbi.nlm.nih.gov/pubmed/24145510,
• Other References: *Gaillard D, Laugerette F, Darcel N, El-Yassimi A, Passilly-Degrace P, Hichami A, Akhtar Khan N, Montmayeur JP, Besnard P: The gustatory pathway is involved in CD36-mediated orosensory perception of long-chain fatty acids in the mouse. FASEB J. 2007 Dec 27, URL: https://www.ncbi.nlm.nih.gov/pubmed/18162488?ordin,*van Oort MM, van Doorn JM, Bonen A, Glatz JF, van der Horst DJ, Rodenburg KW, Luiken JJ: Insulin-induced translocation of CD36 to the plasma membrane is reversible and shows similarity to that of GLUT4. Biochim Biophys Acta. 2007 Dec 15, URL: https://www.ncbi.nlm.nih.gov/pubmed/18167317?ordin,*Won WJ, Bachmann MF, Kearney JF: CD36 Is Differentially Expressed on B Cell Subsets during Development and in Responses to Antigen. J Immunol. 2008 Jan 1180(1):230-7., URL: https://www.ncbi.nlm.nih.gov/pubmed/18097024?ordin,
• Storage Conditions: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.