USP5 antibody

• Background:

  Cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers. Involved in unanchored 'Lys-48'-linked polyubiquitin disassembly. Binds linear and 'Lys-63'-linked polyubiquitin with a lower affinity. Knock-down of USP5 causes the accumulation of p53/TP53 and an increase in p53/TP53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated p53/TP53 but not with MDM2 for proteasomal recognition.

• Synonyms:

  Ubiquitin carboxyl-terminal hydrolase 5|Deubiquitinating enzyme 5|Isopeptidase T|Ubiquitin thioesterase 5|Ubiquitin-specific-processing protease 5|USP5|ISOT

• Gene ID:

  8078

• UniProt:

  P45974

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Ubiquitin specific peptidase 5 (isopeptidase T)

• Target:

  USP5

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Applications:

  ELISA, WB

• Field of Research:

  Epigenetics, Metabolism

• Purification:

  Protein A+G purification

• Dilution:

  WB: 1:500-1:5000

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  93 kDa

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Clone ID:

  6G1

• Tested Applications:

  ELISA, WB