CCN3 antibody

• Background:

  Immediate-early protein playing a role in various cellular processes including proliferation, adhesion, migration, differentiation and survival (PubMed:15181016, PubMed:15611078, PubMed:12695522, PubMed:21344378, PubMed:12050162) . Acts by binding to integrins or membrane receptors such as NOTCH1 (PubMed:12695522, PubMed:21344378, PubMed:15611078) . Essential regulator of hematopoietic stem and progenitor cell function (PubMed:17463287) . Inhibits myogenic differentiation through the activation of Notch-signaling pathway (PubMed:12050162) . Inhibits vascular smooth muscle cells proliferation by increasing expression of cell-cycle regulators such as CDKN2B or CDKN1A independently of TGFB1 signaling (PubMed:20139355) . Ligand of integrins ITGAV:ITGB3 and ITGA5:ITGB1, acts directly upon endothelial cells to stimulate pro-angiogenic activities and induces angiogenesis. In endothelial cells, supports cell adhesion, induces directed cell migration (chemotaxis) and promotes cell survival (PubMed:12695522) . Plays also a role in cutaneous wound healing acting as integrin receptor ligand. Supports skin fibroblast adhesion through ITGA5:ITGB1 and ITGA6:ITGB1 and induces fibroblast chemotaxis through ITGAV:ITGB5. Seems to enhance bFGF-induced DNA synthesis in fibroblasts (PubMed:15611078) . Involved in bone regeneration as a negative regulator (By similarity) . Enhances the articular chondrocytic phenotype, whereas it repressed the one representing endochondral ossification (PubMed:21871891) . Impairs pancreatic beta-cell function, inhibits beta-cell proliferation and insulin secretion (By similarity) . Plays a role as negative regulator of endothelial pro-inflammatory activation reducing monocyte adhesion, its anti-inflammatory effects occur secondary to the inhibition of NF-kappaB signaling pathway (PubMed:21063504) . Contributes to the control and coordination of inflammatory processes in atherosclerosis (By similarity) . Attenuates inflammatory pain through regulation of IL1B-and TNF-induced MMP9, MMP2 and CCL2 expression. Inhibits MMP9 expression through ITGB1 engagement (PubMed:21871891) .

• Synonyms:

  CCN family member 3|Cellular communication network factor 3|Insulin-like growth factor-binding protein 9 (IBP-9, IGF-binding protein 9, IGFBP-9) |Nephro blastoma-overexpressed gene protein homolog|Protein NOV homolog (NovH) |CCN3|IGFBP9|NOV|NOVH

• Gene ID:

  4856

• UniProt:

  P48745

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Nephroblastoma overexpressed gene

• Target:

  CCN3

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  ELISA, WB, IHC

• Field of Research:

  Immunology, Cardiovascular, Signal Transduction

• Purification:

  Immunogen affinity purified

• Dilution:

  WB: 1:500-1:2000; IHC: 1:20-1:200

• Purity:

  ≥95% as determined by SDS-PAGE

• Form:

  Liquid

• Molecular Weight:

  45 kDa

• Shipping Conditions:

  4°C with ice bag

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

• Tested Applications:

  ELISA, WB, IHC