MSH2 antibody

CAT: 0710-FNab05372Size: 100 µgDry Ice: NoHazardous: No
CAT#:0710-FNab05372Size:100 µg
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Background
Component of the post-replicative DNA mismatch repair system (MMR) . Forms two different heterodimers: MutS alpha (MSH2-MSH6 heterodimer) and MutS beta (MSH2-MSH3 heterodimer) which binds to DNA mismatches thereby initiating DNA repair. When bound, heterodimers bend the DNA helix and shields approximately 20 base pairs. MutS alpha recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. MutS beta recognizes larger insertion-deletion loops up to 13 nucleotides long. After mismatch binding, MutS alpha or beta forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. In melanocytes may modulate both UV-B-induced cell cycle regulation and apoptosis.
Synonyms
DNA mismatch repair protein Msh2 (hMSH2) |MutS protein homolog 2|MSH2
Gene ID
4436
UniProt
P43246
Host
Mouse
Reactivity
Human
Immunogen
MutS homolog 2, colon cancer, nonpolyposis type 1
Target
MSH2
Clonality
Monoclonal
Isotype
IgG2a
Applications
ELISA, WB, IHC
Field of Research
Epigenetics, Metabolism, Cancer, Immunology, Developmental biology
Purification
Protein A+G purification
Dilution
WB: 1:500-1:2000; IHC: 1:20-1:200
Purity
≥95% as determined by SDS-PAGE
Form
Liquid
Molecular Weight
105 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months (Avoid repeated freeze / thaw cycles.)
Clone ID
7C1
Tested Applications
ELISA, WB, IHC
Immunogen Sequence
585-934aa

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