Calcein-AM

• Description :

  Calcein AM, has cell membrane permeability and can easily enter the cell. Calcein AM has no fluorescence and is hydrolyzed by endogenous esterase in the cell to produce polar molecule Calcein (Calcein), which has strong negative charge and cannot permeate the cell membrane. Calcein can emit strong green fluorescence, so it is often used with Propidium Iodide for cell viability/virulence detection, excitation/emission wavelength: 494/515 nm[1].

• Product Name Alternative :

  Calcein acetoxymethyl ester

• UNSPSC :

  12171500

• Hazard Statement :

  H302

• Target :

  Fluorescent Dye

• Type :

  Dye Reagents

• Related Pathways :

  Others

• Applications :

  Cancer-programmed cell death

• Field of Research :

  Cancer

• Assay Protocol :

  https://www.medchemexpress.com/Calcein-AM.html

• Purity :

  99.73

• Solubility :

  DMSO : 100 mg/mL (ultrasonic)

• Smiles :

  O=C1OC2(C(C=C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OC(C)=O)=C3)=C3OC4=CC(OC(C)=O)=C(CN(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C24)C5=C1C=CC=C5

• Molecular Formula :

  C46H46N2O23

• Molecular Weight :

  994.86

• Precautions :

  H302

• References & Citations :

  [1]Wang XM, et al. A new microcellular cytotoxicity test based on calcein-AM release. Hum Immunol. 1993 Aug;37 (4) :264-70.|[2]Braut-Boucher F, et al. A non-isotopic, highly sensitive, fluorimetric, cell-cell adhesion microplate assay using calceinAM-labeled lymphocytes. J Immunol Methods. 1995 Jan 13;178 (1) :41-51.|[3]Bratosin D, et al. Novel fluorescence assay using calcein-AM for the determination of human erythrocyte viabilityand aging. Cytometry A. 2005 Jul;66 (1) :78-84.|[4]Holló Z, et al. Calcein accumulation as a fluorometric functional assay of the multidrug transporter. Biochim Biophys Acta. 1994 May 11;1191 (2) :384-8.

• Shipping Conditions :

  Dry Ice

• Storage Conditions :

  -80°C, protect from light, stored under nitrogen

• Scientific Category :

  Dye Reagents

• Clinical Information :

  No Development Reported

• CAS Number :

  [148504-34-1]