CaMK2-beta/gamma/delta (Phospho-Thr287) Colorimetric Cell-Based ELISA Kit

• Description:

  The CaMK2-beta/gamma/delta (Phospho-Thr287) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CaMK2-beta/gamma/delta (Phospho-Thr287) protein expression profile in cells. The kit can be used for measuring the relative amounts of CaMK2-beta/gamma/delta (Phospho-Thr287) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CaMK2-beta/gamma/delta (Phospho-Thr287) .

• Synonyms:

  Calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II subunit beta; CaMK-II subunit beta;2.7.11.17; CAMK2B; CAM2, CAMK2, CAMKB

• Gene Name:

  CAMK2B

• UniProt:

  Q13554

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain. Expressed in skeletal muscle.

• Applications:

  ELISA

• Detection Range:

  > 5000 cells/well

• Function:

  Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca (2+) /calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca (2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca (2+) transport and in fast-twitch muscle participates in the control of Ca (2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2.

• Molecular Weight:

  72678 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Calcium/calmodulin-dependent protein kinase type II subunit beta

• Subcellular Location:

  Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side. In slow- twitch muscle, evenly distributed between longitudinal SR and junctional SR.