MITF (Phospho-Ser180/73) Colorimetric Cell-Based ELISA Kit

• Description:

  The MITF (Phospho-Ser180/73) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor MITF (Phospho-Ser180/73) protein expression profile in cells. The kit can be used for measuring the relative amounts of MITF (Phospho-Ser180/73) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on MITF (Phospho-Ser180/73) .

• Synonyms:

  MITF M1; Microphthalmia-associated transcription factor

• Gene Name:

  MITF

• UniProt:

  O75030

• Reactivity:

  Human, Mouse

• Tissue Specificity:

  Isoform M is exclusively expressed in melanocytes and melanoma cells. Isoform A and isoform H are widely expressed in many cell types including melanocytes and retinal pigment epithelium (RPE) . Isoform C is expressed in many cell types including RPE but not in melanocyte-lineage cells. Isoform Mdel is widely expressed in melanocytes, melanoma cell lines and tissues, but almost undetectable in non-melanoma cell lines.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR) . Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1) . Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.

• Molecular Weight:

  58795 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Microphthalmia-associated transcription factor

• Subcellular Location:

  Nucleus.