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MASTL Colorimetric Cell-Based ELISA

CAT: 0519-EKC1899Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1899Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The MASTL Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor MASTL protein expression profile in cells. The kit can be used for measuring the relative amounts of MASTL in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on MASTL.
Synonyms
Serine/threonine-protein kinase greatwall; GW; GWL; hGWL;2.7.11.1; Microtubule-associated serine/threonine-protein kinase-like; MAST-L; MASTL; GW, GWL, THC2
Gene Name
MASTL
UniProt
Q96GX5
Reactivity
Human, Mouse
Applications
ELISA
Detection Range
> 5000 cells/well
Function
Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation.
Molecular Weight
97319 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Serine/threonine-protein kinase greatwall
Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Nucleus. Cleavage furrow. During interphase is mainly nuclear, upon nuclear envelope breakdown localizes at the cytoplasm and during mitosis at the centrosomes. Upon mitotic exit moves to the cleavage furrow.

UniProtKB · Q96GX5

Serine/threonine-protein kinase greatwall

GWL_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q96GX5
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
MASTL
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
GW, GWL, hGWL
EC number
2.7.11.1
Processing
—
Secondary accessions
Q5T8D5, Q5T8D7, Q8NCD6, Q96SJ5
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationPhosphoprotein

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingNucleotide-binding

Biological process

Cell cycleCell divisionMitosis

Cellular component

CytoplasmCytoskeletonNucleus

Disease

Disease variant

Molecular function

KinaseSerine/threonine-protein kinaseTransferase

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