TRIM24 Colorimetric Cell-Based ELISA

• Description:

  The TRIM24 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor TRIM24 protein expression profile in cells. The kit can be used for measuring the relative amounts of TRIM24 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on TRIM24.

• Synonyms:

  Transcription intermediary factor 1-alpha; TIF1-alpha;2.3.2.27; E3 ubiquitin-protein ligase TRIM24; RING finger protein 82; RING-type E3 ubiquitin transferase TIF1-alpha ; Tripartite motif-containing protein 24; TRIM24; RNF82, TIF1, TIF1A

• Gene Name:

  TRIM24

• UniProt:

  O15164

• Reactivity:

  Human, Mouse

• Applications:

  ELISA

• Detection Range:

  > 5000 cells/well

• Function:

  Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac) . Has E3 protein-ubiquitin ligase activity. Promotes ubiquitination and proteasomal degradation of p53/TP53. Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes (By similarity) .

• Molecular Weight:

  116831 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Transcription intermediary factor 1-alpha

• Subcellular Location:

  Nucleus. Cytoplasm. Colocalizes with sites of active transcription. Detected both in nucleus and cytoplasm in some breast cancer samples. Predominantly nuclear.