SRY Colorimetric Cell-Based ELISA

• Description:

  The SRY Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor SRY protein expression profile in cells. The kit can be used for measuring the relative amounts of SRY in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on SRY.

• Synonyms:

  Sex-determining region Y protein; Testis-determining factor; SRY; TDF

• Gene Name:

  SRY

• UniProt:

  Q05066

• Reactivity:

  Human

• Applications:

  ELISA

• Detection Range:

  > 5000 cells/well

• Function:

  Transcriptional regulator that controls a genetic switch in male development. It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells (By similarity) . In male adult brain involved in the maintenance of motor functions of dopaminergic neurons (By similarity) . Involved in different aspects of gene regulation including promoter activation or repression (By similarity) . Promotes DNA bending. SRY HMG box recognizes DNA by partial intercalation in the minor groove. Also involved in pre-mRNA splicing. Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3'.

• Molecular Weight:

  23884 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Sex-determining region Y protein

• Subcellular Location:

  Nucleus speckle. Cytoplasm. Nucleus. Acetylation contributes to its nuclear localization and deacetylation by HDAC3 induces a cytoplasmic delocalization (PubMed:15297880) . Colocalizes with SOX6 in speckles (PubMed:11818535) . Colocalizes with CAML in the nucleus (PubMed:15746192) . Colocalizes in the nucleus with ZNF208 isoform KRAB-O and tyrosine hydroxylase (TH) (By similarity) .