PPIF Colorimetric Cell-Based ELISA

• Description:

  The PPIF Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PPIF protein expression profile in cells. The kit can be used for measuring the relative amounts of PPIF in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PPIF.

• Synonyms:

  Peptidyl-prolyl cis-trans isomerase F, mitochondrial; PPIase F;5.2.1.8; Cyclophilin D; CyP-D; CypD; Cyclophilin F; Mitochondrial cyclophilin; CyP-M; Rotamase F; PPIF; CYP3

• Gene Name:

  PPIF

• UniProt:

  P30405

• Reactivity:

  Human, Mouse, Rat

• Applications:

  ELISA

• Detection Range:

  > 5000 cells/well

• Function:

  PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Involved in regulation of the mitochondrial permeability transition pore (mPTP) . It is proposed that its association with the mPTP is masking a binding site for inhibiting inorganic phosphate (Pi) and promotes the open probability of the mPTP leading to apoptosis or necrosis; the requirement of the PPIase activity for this function is debated. In cooperation with mitochondrial TP53 is involved in activating oxidative stress- induced necrosis. Involved in modulation of mitochondrial membrane F (1) F (0) ATP synthase activity and regulation of mitochondrial matrix adenine nucleotide levels. Has anti-apoptotic activity independently of mPTP and in cooperation with BCL2 inhibits cytochrome c-dependent apoptosis.

• Molecular Weight:

  22040 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Peptidyl-prolyl cis-trans isomerase F, mitochondrial

• Subcellular Location:

  Mitochondrion matrix.