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CALR Colorimetric Cell-Based ELISA

CAT: 0519-EKC1701Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1701Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The CALR Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CALR protein expression profile in cells. The kit can be used for measuring the relative amounts of CALR in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CALR.
Synonyms
Calreticulin; CRP55; Calregulin; Endoplasmic reticulum resident protein 60; ERp60; HACBP; grp60; CALR; CRTC
Gene Name
CALR
UniProt
P27797
Reactivity
Human, Mouse, Rat
Applications
ELISA
Detection Range
> 5000 cells/well
Function
Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity) .
Molecular Weight
48142 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Calreticulin
Subcellular Location
Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface. Sarcoplasmic reticulum lumen. Also found in cell surface (T cells), cytosol and extracellular matrix (PubMed:10358038) . Associated with the lytic granules in the cytolytic T-lymphocytes.

UniProtKB · P27797

Calreticulin

CALR_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P27797
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CALR
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q6IAT4, Q9UDG2
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationDisulfide bondGlycoproteinHydroxylation

Ligand

CalciumLectinMetal-bindingZinc

Molecular function

Chaperone

Cellular component

CytoplasmCytoplasmic vesicleEndoplasmic reticulumExtracellular matrixLysosomeSarcoplasmic reticulumSecreted

Domain

RepeatSignal

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