ZIC1/2/3 Colorimetric Cell-Based ELISA

• Description:

  The ZIC1/2/3 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor ZIC1/2/3 protein expression profile in cells. The kit can be used for measuring the relative amounts of ZIC1/2/3 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on ZIC1/2/3.

• Synonyms:

  Zinc finger protein ZIC 1; Zinc finger protein 201; Zinc finger protein of the cerebellum 1; ZIC1; ZIC, ZNF201

• Gene Name:

  ZIC1

• UniProt:

  Q15915

• Reactivity:

  Human, Mouse

• Tissue Specificity:

  CNS. A high level expression is seen in the cerebellum. Detected in the nuclei of the cerebellar granule cell lineage from the progenitor cells of the external germinal layer to the postmigrated cells of the internal granular layer. Detected in medulloblastoma (26/29 cases), but not present in all other tumors examined.

• Applications:

  ELISA

• Detection Range:

  > 5000 cells/well

• Function:

  Acts as a transcriptional activator. Involved in neurogenesis. Plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. Involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN) . Plays a role in the regulation of MF axon pathway choice. Promotes MF migration towards ipsilaterally- located cerebellar territories. May have a role in shear flow mechanotransduction in osteocytes. Retains nuclear GLI1 and GLI3 in the cytoplasm. Binds to the minimal GLI-consensus sequence 5'- TGGGTGGTC-3' (By similarity) .

• Molecular Weight:

  48309 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Zinc finger protein ZIC 1

• Subcellular Location:

  Nucleus. Cytoplasm. Localizes in the cytoplasm in presence of MDFIC overexpression.