Smad2 (Phospho-Ser250) Colorimetric Cell-Based ELISA Kit

• Description:

  The Smad2 (Phospho-Ser250) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Smad2 (Phospho-Ser250) protein expression profile in cells. The kit can be used for measuring the relative amounts of Smad2 (Phospho-Ser250) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Smad2 (Phospho-Ser250) .

• Synonyms:

  JV18-1; MADH2; MADR2; Mad-related protein 2; Mothers against DPP homolog 2; Mothers against decapentaplegic homolog 2; Smad 2

• Gene Name:

  SMAD2

• UniProt:

  Q15796

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

• Molecular Weight:

  52306 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Mothers against decapentaplegic homolog 2

• Subcellular Location:

  Cytoplasm. Nucleus. Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:9865696) . On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081) .