Products for Research Use Only
RegisterLogin

PSEN1 Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1487Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0519-EKC1487Size:1 Kit, containing one 96 Well Plate and all necessary reagents
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
The PSEN1 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor PSEN1 protein expression profile in cells. The kit can be used for measuring the relative amounts of PSEN1 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on PSEN1.
Synonyms
Presenilin-1; PS-1;3.4.23.- ; Protein S182; Presenilin-1 NTF subunit; Presenilin-1 CTF subunit; Presenilin-1 CTF12; PS1-CTF12; PSEN1; AD3, PS1, PSNL1
Gene Name
PSEN1
UniProt
P49768
Reactivity
Human
Tissue Specificity
Detected in azurophile granules in neutrophils and in platelet cytoplasmic granules (at protein level) (PubMed:11987239) . Expressed in a wide range of tissues including various regions of the brain, liver, spleen and lymph nodes (PubMed:7596406, PubMed:8641442, PubMed:8574969) .
Applications
ELISA
Detection Range
> 5000 cells/well
Function
Catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed:15274632, PubMed:10545183, PubMed:10593990, PubMed:10206644, PubMed:10899933, PubMed:10811883, PubMed:12679784, PubMed:12740439, PubMed:25043039, PubMed:26280335) . Requires the presence of the other members of the gamma-secretase complex for protease activity (PubMed:15274632, PubMed:25043039, PubMed:26280335) . Plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (PubMed:9738936, PubMed:10593990, PubMed:10899933, PubMed:10811883) . Stimulates cell-cell adhesion via its interaction with CDH1; this stabilizes the complexes between CDH1 (E-cadherin) and its interaction partners CTNNB1 (beta-catenin), CTNND1 and JUP (gamma-catenin) (PubMed:11953314) . Under conditions of apoptosis or calcium influx, cleaves CDH1 (PubMed:11953314) . This promotes the disassembly of the complexes between CDH1 and CTNND1, JUP and CTNNB1, increases the pool of cytoplasmic CTNNB1, and thereby negatively regulates Wnt signaling (PubMed:9738936, PubMed:11953314) . Required for normal embryonic brain and skeleton development, and for normal angiogenesis (By similarity) .
Molecular Weight
52668 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Presenilin-1
Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic granule. Cell membrane. Translocates with bound NOTCH1 from the endoplasmic reticulum and/or Golgi to the cell surface (PubMed:10593990) . Colocalizes with CDH1/2 at sites of cell-cell contact. Colocalizes with CTNNB1 in the endoplasmic reticulum and the proximity of the plasma membrane (PubMed:9738936) . Also present in azurophil granules of neutrophils (PubMed:11987239) . Colocalizes with UBQLN1 in the cell membrane and in cytoplasmic juxtanuclear structures called aggresomes (PubMed:21143716) .

UniProtKB · P49768

Presenilin-1

PSN1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P49768
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PSEN1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
PS-1
EC number
3.4.23.-
Processing
—
Secondary accessions
B2R6D3, O95465, Q14762, Q15719, Q15720, Q96P33, Q9UIF0
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Disease

Alzheimer diseaseAmyloidosisCardiomyopathyDisease variantNeurodegeneration

Biological process

ApoptosisCell adhesionNotch signaling pathway

Cellular component

Cell membraneCell projectionEndoplasmic reticulumEndosomeGolgi apparatusMembraneSynapse

Molecular function

HydrolaseProtease

PTM

Phosphoprotein

Domain

TransmembraneTransmembrane helix

Related Products

CatalogName
EKC2622PSEN1 (Phospho-Ser357) Colorimetric Cell-Based ELISA Kit
EKC1359MEF2A Colorimetric Cell-Based ELISA Kit
EKC1455PHCA Colorimetric Cell-Based ELISA Kit
EKC1415Osteopontin Colorimetric Cell-Based ELISA Kit
EKC1373MKP1 Colorimetric Cell-Based ELISA Kit
EKC1112Chk1 Colorimetric Cell-Based ELISA Kit
EKC1526Smad1 Colorimetric Cell-Based ELISA Kit
EKC1555Synaptophysin Colorimetric Cell-Based ELISA Kit
EKC1598WAVE1 Colorimetric Cell-Based ELISA Kit
EKC1044ATF7 Colorimetric Cell-Based ELISA Kit

Alternative Products

CatalogName
MBS9500510-01PSEN1 Colorimetric Cell-Based ELISA Kit
OKAG00999PSEN1 Colorimetric Cell-Based ELISA Kit (OKAG00999)
MBS9501684-01Phospho-PSEN1 (Ser357) Colorimetric Cell-Based ELISA Kit
OKAG02174Phospho-PSEN1 (Ser357) Colorimetric Cell-Based ELISA Kit (OKAG02174)
CB5592PSEN1 Cell-Based ELISA Kit
CBP1835Phospho-PSEN1 (Ser357) Cell-Based ELISA Kit
MBS9500271-01HBP1 Colorimetric Cell-Based ELISA Kit
MBS9500106-01CD5 Colorimetric Cell-Based ELISA Kit
MBS9500261-01GR Colorimetric Cell-Based ELISA Kit
MBS9500259-01GLUT3 Colorimetric Cell-Based ELISA Kit

Popular Products