CREB-BP Colorimetric Cell-Based ELISA Kit

• Description:

  The CREB-BP Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CREB-BP protein expression profile in cells. The kit can be used for measuring the relative amounts of CREB-BP in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CREB-BP.

• Synonyms:

  CREB-binding protein; CREBBP; CBP

• Gene Name:

  CREBBP

• UniProt:

  Q92793

• Reactivity:

  Human, Mouse

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Acetylates histones, giving a specific tag for transcriptional activation. Also acetylates non-histone proteins, like NCOA3 and FOXO1. Binds specifically to phosphorylated CREB and enhances its transcriptional activity toward cAMP-responsive genes. Acts as a coactivator of ALX1. Acts as a circadian transcriptional coactivator which enhances the activity of the circadian transcriptional activators: NPAS2-ARNTL/BMAL1 and CLOCK- ARNTL/BMAL1 heterodimers. Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (PubMed:24939902) .

• Molecular Weight:

  265351 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  CREB-binding protein

• Subcellular Location:

  Cytoplasm. Nucleus. Recruited to nuclear bodies by SS18L1/CREST. In the presence of ALX1 relocalizes from the cytoplasm to the nucleus.