CaMK4 Colorimetric Cell-Based ELISA Kit

• Description:

  The CaMK4 Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CaMK4 protein expression profile in cells. The kit can be used for measuring the relative amounts of CaMK4 in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CaMK4.

• Synonyms:

  Calcium/calmodulin-dependent protein kinase type IV catalytic chain; Calspermin; CAM kinase-GR; CaMK IV; CAMK4; CaMKIV; KCC4; kinase CaMK4

• Gene Name:

  CAMK4

• UniProt:

  Q16566

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Expressed in brain, thymus, CD4 T-cells, testis and epithelial ovarian cancer tissue.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Calcium/calmodulin-dependent protein kinase that operates in the calcium-triggered CaMKK-CaMK4 signaling cascade and regulates, mainly by phosphorylation, the activity of several transcription activators, such as CREB1, MEF2D, JUN and RORA, which play pivotal roles in immune response, inflammation, and memory consolidation. In the thymus, regulates the CD4 (+) /CD8 (+) double positive thymocytes selection threshold during T-cell ontogeny. In CD4 memory T-cells, is required to link T-cell antigen receptor (TCR) signaling to the production of IL2, IFNG and IL4 (through the regulation of CREB and MEF2) . Regulates the differentiation and survival phases of osteoclasts and dendritic cells (DCs) . Mediates DCs survival by linking TLR4 and the regulation of temporal expression of BCL2. Phosphorylates the transcription activator CREB1 on 'Ser-133' in hippocampal neuron nuclei and contribute to memory consolidation and long term potentiation (LTP) in the hippocampus. Can activate the MAP kinases MAPK1/ERK2, MAPK8/JNK1 and MAPK14/p38 and stimulate transcription through the phosphorylation of ELK1 and ATF2. Can also phosphorylate in vitro CREBBP, PRM2, MEF2A and STMN1/OP18.

• Molecular Weight:

  51926 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  Calcium/calmodulin-dependent protein kinase type IV

• Subcellular Location:

  Cytoplasm. Nucleus. Localized in hippocampal neuron nuclei. In spermatids, associated with chromatin and nuclear matrix (By similarity) .