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Caldesmon Colorimetric Cell-Based ELISA Kit

CAT: 0519-EKC1073Size: 1 Kit, containing one 96 Well Plate and all necessary reagentsDry Ice: NoHazardous: No
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CAT#:0519-EKC1073Size:1 Kit, containing one 96 Well Plate and all necessary reagents
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The Caldesmon Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor Caldesmon protein expression profile in cells. The kit can be used for measuring the relative amounts of Caldesmon in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on Caldesmon.
Synonyms
CAD; CALD1; CDM; L-caldesmon; Non-muscle caldesmon
Gene Name
CALD1
UniProt
Q05682
Reactivity
Human, Mouse, Rat
Tissue Specificity
High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular- weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.
Applications
ELISA
Sample Type
Cell lines
Detection Range
> 5000 cells/well
Function
Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments) . Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity) .
Molecular Weight
93231 MW
Shipping Conditions
Available
Storage Conditions
Store at 4°C for up to 6 months.
Other Gene Names
Caldesmon
Subcellular Location
Cytoplasm, cytoskeleton. Cytoplasm, myofibril. On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle) .

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