C/EBP-beta Colorimetric Cell-Based ELISA Kit

• Description:

  The C/EBP-beta Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor C/EBP-beta protein expression profile in cells. The kit can be used for measuring the relative amounts of C/EBP-beta in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on C/EBP-beta.

• Synonyms:

  AGP/EBP; C/EBP beta; C/EBP-related protein 2; CCAAT/enhancer binding protein beta; CEBPB; CRP2; IL-6DBP; Interleukin-6- dependent binding protein; LAP; Liver-enriched transcriptional activator; Nuclear factor NF-IL6; SF-B; SFB; Silencer factor B; TCF5; Transcription factor 5

• Gene Name:

  CEBPB PP9092

• UniProt:

  P17676

• Reactivity:

  Human, Mouse, Rat

• Tissue Specificity:

  Expressed at low levels in the lung, kidney and spleen.

• Applications:

  ELISA

• Sample Type:

  Cell lines

• Detection Range:

  > 5000 cells/well

• Function:

  Important transcription factor regulating the expression of genes involved in immune and inflammatory responses (PubMed:1741402, PubMed:9374525, PubMed:12048245, PubMed:18647749) . Plays also a significant role in adipogenesis, as well as in the gluconeogenic pathway, liver regeneration, and hematopoiesis. The consensus recognition site is 5'- T[TG]NNGNAA[TG]-3'. Its functional capacity is governed by protein interactions and post-translational protein modifications. During early embryogenesis, plays essential and redundant functions with CEBPA. Has a promitotic effect on many cell types such as hepatocytes and adipocytes but has an antiproliferative effect on T-cells by repressing MYC expression, facilitating differentiation along the T-helper 2 lineage. Binds to regulatory regions of several acute-phase and cytokines genes and plays a role in the regulation of acute-phase reaction and inflammation. Plays also a role in intracellular bacteria killing (By similarity) . During adipogenesis, is rapidly expressed and, after activation by phosphorylation, induces CEBPA and PPARG, which turn on the series of adipocyte genes that give rise to the adipocyte phenotype. The delayed transactivation of the CEBPA and PPARG genes by CEBPB appears necessary to allow mitotic clonal expansion and thereby progression of terminal differentiation (PubMed:20829347) . Essential for female reproduction because of a critical role in ovarian follicle development (By similarity) . Restricts osteoclastogenesis (By similarity) .

• Molecular Weight:

  36106 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 4°C for up to 6 months.

• Other Gene Names:

  CCAAT/enhancer-binding protein beta

• Subcellular Location:

  Nucleus. Cytoplasm. Translocates to the nucleus when phosphorylated at Ser-288. In T-cells when sumoylated drawn to pericentric heterochromatin thereby allowing proliferation (By similarity) .