Anti-Phospho-AFX (Ser197) FOXO4 Antibody

• Background:

  Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.
  
  Di Maira G, et al. (2005)Cell Death Differ; 12(6): 668-77.
  Essers MA, et al. EMBO J 2004 Nov. 11.
  Brownawell AM, (2001) Mol Cell Biol; 21(10): 3534-46.
  Kops GJ, et al. (1999) Nature; 398(6728): 630-4.

• Description:

  Boster Bio Anti-Phospho-AFX (Ser197) FOXO4 Antibody (Catalog # P01819) . Tested in WB, IHC, IF applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  Forkhead box protein O4; Fork head domain transcription factor AFX1; FOXO4; AFX, AFX1, MLLT7

• Gene Name:

  FOXO4

• UniProt:

  P98177

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  Peptide sequence around phosphorylation site of serine 197 (A-A-S (p) -M-D) derived from Human AFX.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.

• Applications:

  WB, IHC, IF

• Field of Research:

  Domain Families, Epigenetics and Nuclear Signaling, Forkhead Box, Transcription

• Purification:

  Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/ml

• Form:

  Liquid

• Function:

  Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity. .

• Molecular Weight:

  53684 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Other Gene Names:

  Forkhead box protein O4

• Subcellular Location:

  Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.