Anti-FoxO4/Afx Rabbit Monoclonal Antibody

• Description:

  Boster Bio Anti-FoxO4/Afx Rabbit Monoclonal Antibody catalog # M01819-1. Tested in WB, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  Forkhead box protein O4; Fork head domain transcription factor AFX1; FOXO4; AFX, AFX1, MLLT7

• Gene Name:

  Forkhead box protein O4

• UniProt:

  P98177

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthesized peptide derived from human FoxO4

• Clonality:

  Monoclonal

• Clone:

  Clone: HEH-6

• Tissue Specificity:

  Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Isoform zeta is most abundant in the liver, kidney, and pancreas.

• Applications:

  WB, ICC, IF, IP, Flow Cytometry

• Field of Research:

  Domain Families, Epigenetics and Nuclear Signaling, Forkhead Box, Transcription

• Purification:

  Affinity-chromatography

• Concentration:

  Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Reconstitution:

  Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

• Function:

  Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle. Involved in increased proteasome activity in embryonic stem cells (ESCs) by activating expression of PSMD11 in ESCs, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity. .

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  WB 1:1000-1:2000
  ICC/IF 1:50-1:200
  IP 1:50
  FC 1:100

• Other Gene Names:

  FOXO4

• Subcellular Location:

  Cytoplasm. Nucleus. When phosphorylated, translocated from nucleus to cytoplasm. Dephosphorylation triggers nuclear translocation. Monoubiquitination increases nuclear localization. When deubiquitinated, translocated from nucleus to cytoplasm.

• Protein Name:

  Lysosome-associated membrane glycoprotein 2 (Lamp2)