AFX (phospho Ser197) Antibody

• Background:

  Transcription factor involved in the regulation of the insulin signaling pathway. Binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. Down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. Also involved in negative regulation of the cell cycle.

• NCBI Gene ID:

  4303

• Swiss Prot:

  P98177

• Accession Number:

  NP_001164402.1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  AFX (Phospho-Ser197) antibody was raised against a peptide sequence around phosphorylation site of serine 197 (A-A-S (p) -M-D) derived from Human AFX.

• Clonality:

  Polyclonal

• Conjugation:

  Unconjugated

• Type:

  Primary Antibodies

• Field of Research:

  Phospho-Specific, Apoptosis, Cancer

• Purification:

  Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

• Concentration:

  1 mg/mL

• Buffer:

  Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

• Modification:

  Phospho-Specific

• Shipping Conditions:

  Blue Ice

• Storage Conditions:

  Store antibody at -20˚ C for up to one year.

• Calculated Molecular Weight:

  65 kDa

• Specificity:

  This antibody detects endogenous level of AFX only when phosphorylated at serine 197.

• Applications Notes:

  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200

• Symbol:

  FOXO4

• NCBI Official Name:

  Forkhead box O4

• NCBI Organism:

  Homo sapiens

• Background Reference 01:

  Di Maira G, et al. (2005) Cell Death Differ; 12 (6) : 668-77. Essers MA, et al. EMBO J 2004 Nov. 11. Brownawell AM, (2001) Mol Cell Biol; 21 (10) : 3534-46. Kops GJ, et al. (1999) Nature; 398 (6728) : 630-4.

• Other Product Names:

  AFX, AFX1, MLLT7, EIF2A, AFX, FOXO4, Afxh

• Tested Applications:

  WB, IHC, IF

• Protein ID:

  283436083

• Physical Properties:

  Liquid