Anti-N-Cadherin-2 CDH2 CD325-Monoclonal Antibody

• Background:

  Recognizes a protein of ~140kDa, identified as N-Cadherin (NCAD), also known as CD325. N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. Expression of N-cadherin has been reported on a variety of normal tissues including neuronal, endothelial and muscle cells, and a subpopulation of early hematopoietic progenitor cells. Results aid in the classification of malignant non-carcinomatous neoplasms including mesotheliomas, chordomas, synovial sarcomas, malignant melanomas, epithelioid sarcomas, epithelioid angiosarcomas, clear cell sarcomas as well as serous and endometrioid tumors of the ovary have been demonstrated to be N-cadherin positive, whereas mucinous tumors are negative. Other N-cadherin-positive neoplasms include renal cell carcinomas and some variant breast tumors, including medullary breast carcinomas and sarcomatoid metaplastic breast carcinomas.

• Description:

  Boster Bio Anti-N-Cadherin-2 CDH2 CD325-Monoclonal Antibody (Catalog # M01577) . Tested in IHC applications. This antibody reacts with Human, Mouse.

• Synonyms:

  Cadherin-2; CDw325; Neural cadherin; N-cadherin; CD325; CDH2; CDHN, NCAD

• Gene Name:

  CDH2

• UniProt:

  P19022

• Host:

  Mouse

• Reactivity:

  Human, Mouse

• Immunogen:

  Recombinant human CDH2 intracellular domain

• Clonality:

  Monoclonal

• Clone:

  Clone: Clone: CDH2/1573

• Applications:

  IHC

• Field of Research:

  Cadherins, Cell Adhesion, Cytoskeleton/ECM, Signal Transduction

• Purification:

  200ug/ml of antibody purified from Bioreactor Concentrate by Protein A/G.

• Concentration:

  Purified antibody with BSA and azide at 200ug/ml

• Dilution:

  Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.

• Form:

  Liquid

• Function:

  Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity) . .

• Molecular Weight:

  99809 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

• Fragment:

  IgG1, kappa

• Other Gene Names:

  Cadherin-2

• Subcellular Location:

  Cell surface