Anti-Rhodopsin Monoclonal Antibody

• Background:

  Rhodopsin consists of the protein moiety opsin and a reversibly covalently bound cofactor, retinal. Opsin, a bundle of seven membrane embedded alpha-helices, binds retinal, a photo reactive chromophore, in a central pocket (2, 3) . In addition to being the pigment of the retina that is responsible for both the formation of the photoreceptor cells, its function is to specifically convey information stored in the specific geometry of the chormophore to the surface of the molecule upon light absorption (2) . In the active state, rhodopsin activates transduction, a GTP binding protein. Once activated, transduction promotes the hydrolysis of cGMP by phosphodiesterase. Rhodopsin's activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin (4) . Mutations in the rhodopsin gene lead to retinitis pigmentosa, which can be inherited as an autosomal dominant, an autosomal recessive or an X-linked recessive disorder (5) .

• Description:

  Boster Bio Anti-Rhodopsin Monoclonal Antibody catalog # M00083-2. Tested in ELISA, IP, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  OPN2, opsd, opsin 2, opsin 2 rod pigment, opsin2, RHO, RP4, MGC138309, Retinitis Pigmentosa 4

• Gene Name:

  RHO

• UniProt:

  P02699

• Host:

  Mouse

• Reactivity:

  Vertebrates

• Cross Reactivity:

  Detects ~40kDa. Recognizes Rhodopsin (native and recombinant forms) . No known reactivity to other proteins. Binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A- (COOH) .

• Immunogen:

  Bovine Rhodopsin

• Clonality:

  Monoclonal

• Clone:

  Clone: 1D4

• Applications:

  WB, IHC, ICC, IF, IP, ELISA

• Field of Research:

  G Protein Signaling, Neuroscience, Sensory System, Signal Transduction, Signaling Pathway, Visual System

• Purification:

  Protein G Purified

• Concentration:

  1 mg/ml

• Dilution:

  WB (1:1000), IHC (1:100) ; optimal dilutions for assays should be determined by the user.

• Form:

  Liquid

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

• Fragment:

  IgG1

• Subcellular Location:

  Membrane

• Protein Name:

  Rhodopsin