Anti-Rhodopsin Antibody

Anti-Rhodopsin Antibody

• Description: Mouse Monoclonal Antibody specific to Rhodopsin
• Product Name Alternative: Anti-Rhodopsin Mouse Monoclonal Antibody
• CAS Number: 9007-83-4
• Gene Name: RHO
• NCBI Gene ID: RHO
• UniProt: P02699
• Cellular Locus: Membrane, Cell projection, cilium, photoreceptor outer segment
• Host: Mouse
• Reactivity: Vertebrate
• Immunogen: Bovine rhodopsin
• Target Antigen: Rhodopsin
• Target: Rhodopsin
• Clonality: Monoclonal
• Isotype: IgG1
• Type: Antibody
• Applications: WB, IHC, ICC/IF, IP, ELISA
• Field of Research: Neuroscience
• Purification Method: Purified by Protein G affinity chromatography
• Concentration: Lot Specific
• Dilution: Dilute in PBS or medium that is identical to that used in the assay system.
• Format: Purified
• Form: Liquid
• Buffer: Phosphate Buffered Saline
• Function: Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth (By similarity). Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins (PubMed:10926528, PubMed:12044163, PubMed:11972040, PubMed:16908857, PubMed:16586416, PubMed:17060607, PubMed:17449675, PubMed:18818650, PubMed:21389983, PubMed:22198838, PubMed:23579341, PubMed:25205354, PubMed:27458239). Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:1396673, PubMed:15111114). {UniProtKB:P08100, PubMed:1396673, PubMed:16586416, PubMed:16908857, PubMed:17060607, PubMed:17449675, PubMed:18818650, PubMed:21389983, PubMed:22198838, PubMed:23579341, PubMed:25205354, PubMed:27458239, PubMed:10926528, PubMed:11972040, PubMed:12044163, PubMed:15111114, PubMed:26526852}.
• Additionnal Information: Immunoblotting: use at 1-10ug/mL. A band of ~40kDa is detected.Immunohistochemistry/immunocyto- chemistry: use at 1-10ug/mL
  
  Immunoprecipitation: use at 1-2ug per 100-500ug of protein.
  
  These are recommended concentrations. User should determine optimal concentrations for their application.
  
  Positive control: Rat eye lysate
• Storage Conditions: This antibody is stable for at least one (1) year at -20°C.
• Specificity: This antibody recognizes the C-terminal aa TETSQVAPA(COOH) of native and recombinant vertebrate rhodopsin.
• Formulation: PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.
• Buffer pH: pH 7.4
• Target Background: Rhodopsin is comprised of the protein opsin and a reversibly covalently bound cofactor, retinal. Opsin, made up of seven membrane-embedded alpha helices, binds retinal, a photoreactive chromophore, in a central pocket. In addition to being the pigment of the retina that is responsible for the formation of photoreceptor cells, rhodopsin conveys information stored in the chromophore to the surface of the molecule upon light absorption. Mutations in the rhodopsin gene lead to retinitis pigmentosa.