Anti-Bach2 Antibody

• Background:

  This antibody is suitable for Cancer and DNA Damage & Repair research. During an immune response to infectious diseases and cancer, B-cells undergo activation in response to antigen, and differentiate either into short-lived plasma cells, whose numbers contract following withdrawal of the antigenic stimulus, or into memory B-cells. The formation of B-cell memory is the major goal of vaccination, since memory B-cells are able to produce antibodies specific to infections and cancer that can last for the lifetime of the host. Recently it has been shown that the formation of long-lived memory B-cells is dependent upon the transcription factor Bach2, since knockout mice lacking the gene fail to generate class-switched IgG antibody responses and all B-cells undergo default plasma cell differentiation upon antigenic stimulation. While the mechanism by which Bach2 controls B-cell memory fate has been elucidated (it binds and represses the promoter of the gene that encodes the plasma cell master regulatory transcription factor Blimp-1), the upstream mechanism by which the function of Bach2 is regulated is unknown. There is evidence suggesting that Bach2 is phosphorylated in B-cells following stimulation and suspect that this modification may allow the B-cell signaling apparatus to control Bach2 activity and therefore memory fate decisions. Anti-Bach2 is ideal for researchers interested in Cancer and DNA Damage & Repair research.

• Description:

  Boster Bio Anti-Bach2 Antibody (Catalog # A04096) . Tested in ELISA, IP, WB applications. This antibody reacts with Mouse.

• Synonyms:

  Bach2, Bach-2, Bach 2, BTB and CNC homology 1, basic leucine zipper transcription factor 2

• Gene Name:

  BACH2

• UniProt:

  A2RRI0

• Host:

  Rabbit

• Reactivity:

  Mouse

• Immunogen:

  Anti-Bach2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the n-terminus region of the Bach2 protein.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Expression restricted to monocytes and neuronal cells.

• Applications:

  ELISA, IP, WB

• Field of Research:

  Adaptive Immunity, B Cells, Cell Biology, Domain Families, Epigenetics and Nuclear Signaling, Hlh/Leucine Zipper, Immunology, Metabolism, Non-CD, Oxidative Stress, Pathways and Processes, Redox Metabolism, Transcription, Transcription Factors

• Purification:

  Bach2 antibody is directed against mouse Bach2 (n-terminus) protein. Anti-Bach-2 was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest 100% homology with Bach2 protein from mouse. Reactivity against homologues from other sources is not known.

• Concentration:

  0.97mg/ml by UV absorbance at 280 nm

• Form:

  Liquid (sterile filtered)

• Function:

  Transcriptional regulator that acts as repressor or activator. Binds to Maf recognition elements (MARE) . Play important roles in coordinating transcription activation and repression by MAFK.

• Molecular Weight:

  91836 MW

• Shipping Conditions:

  Due to dry ice shipping issues, this product is temporarily not available to distributors outside of the United States.

• Storage Conditions:

  Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

• Fragment:

  IgG

• Applications Notes:

  Affinity purified Bach-2 antibody has been tested for use in ELISA, Immunoprecipitation, and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 91-140 kDa in size corresponding to Bach2 protein by western blotting in the appropriate cell lysate or extract.

• Other Gene Names:

  Transcription regulator protein BACH2

• Subcellular Location:

  Cytoplasm. Nucleus. Nucleocytoplasmic shuttling is controlled by phosphorylation.