Anti-USP36 Antibody

• Description:

  Boster Bio Anti-USP36 Antibody catalog # A07184-2. Tested in WB applications. This antibody reacts with Human, Rat.

• Synonyms:

  Ubiquitin carboxyl-terminal hydrolase 36 ;3.4.19.12; Deubiquitinating enzyme 36; Ubiquitin thioesterase 36; Ubiquitin-specific-processing protease 36; USP36

• Gene Name:

  USP36

• UniProt:

  Q9P275

• Host:

  Rabbit

• Reactivity:

  Human, Rat

• Immunogen:

  Synthetic peptide, corresponding to amino acids 500-550 of Human USP36.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Broadly expressed.

• Applications:

  WB

• Purification:

  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

• Dilution:

  WB: 1:500-1:1000

• Form:

  Liquid

• Function:

  Deubiquitinase essential for the regulation of nucleolar structure and function. Required for cell and organism viability. Plays an important role in ribosomal RNA processing and protein synthesis, which is mediated, at least in part, through deubiquitination of DHX33, NPM1 and FBL, regulating their protein stability (PubMed:29273634, PubMed:19208757, PubMed:22902402) . Function as a transcriptional repressor by deubiquiting histone H2B at the promoters of genes critical for cellular differentiation, such as CDKN1A, thereby preventing histone H3 'Lys-4' trimethylation (H3K4) (PubMed:29274341) . Specifically deubiquitinates MYC in the nucleolus, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 3 of FBXW7 (FBW7gamma) in the nucleolus and counteracting ubiquitination of MYC by the SCF (FBW7) complex. In contrast, it does not interact with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm (PubMed:25775507) . Interacts to and regulates the actions of E3 ubiquitin-protein ligase NEDD4L over substrates such as NTRK1, KCNQ2 and KCNQ3, affecting their expression an functions (PubMed:27445338) . Deubiquitinates SOD2, regulates SOD2 protein stability (PubMed:21268071) . Deubiquitinase activity is required to control selective autophagy activation by ubiquitinated proteins (PubMed:22622177) .

• Molecular Weight:

  122908 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Other Gene Names:

  Ubiquitin carboxyl-terminal hydrolase 36

• Subcellular Location:

  Nucleus, nucleolus

• Protein Name:

  Ubiquitin carboxyl-terminal hydrolase 36