Caspase 4 Activity Detection Substrate for Flow Cytometry

• Type:

  Apoptosis / Caspase

• Detection Method:

  Fluorometric method; Flow cytometry

• Assay Principle:

  The Caspase 4 Activity Detection Substrate for Flow Cytometry are a novel fluorogenic dye that couple the caspase 4 recognition sequence (LEVD) to a high affinity DNA dye, which have cell membrane permeability and can penetrate the plasma membrane into the cytoplasm. The substrate itself has no fluorescence and has a charge exclusion effect with DNA. During cell apoptosis, caspase-4 cleaves the substrate and releases high affinity DNA dyes, which bind to DNA to produce strong fluorescence, thereby detecting caspase-4 activity and visualizing the morphological changes of the cell nucleus during apoptosis.

• Assay Performance Time:

  30 min

• Sample Type:

  Cell samples

• Shipping Conditions:

  Ice Bag

• Storage Conditions:

  This product can be stored at -20°C for 1 year in the dark.

• Detection Instrument:

  Flow Cytometer; Fluorescence microscope