Sheep anti Human chx 10 (Visual system homeobox 2) (NT)

• Background:

  Chx10 is a 46kDa homeodomain protein of the paired-like class that is essential for development of the mammalian eye. Mutations in Chx10 cause microphthalmia, a cause of congenital blindness in Humans, and the ocular retardation (or) phenotype in mice. In the developing mouse retina Chx10 is expressed in retinal progenitors, while in the mature retina, Chx10 expression becomes restricted to bipolar neurons. Concurrent with these expression patterns, the Chx10-/- (or) retina is thin due to a defect in proliferation of retinal progenitors, and lacks bipolar neurons. Chx10 is also expressed in the developing brainstem, thalamus, and spinal cord.

• Synonyms:

  Ceh-10 homeoDomain-containing homolog

• UniProt:

  P58304

• Host:

  Sheep

• Species Reactivity:

  Human, Mouse, Rat, Chicken

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Source:

  Antibody raised in sheep against a recombinant protein corresponding to amino acids 1 to 131 derived from the N-terminal region of the Human Chx10 protein conjugated to KLH.

• Applications:

  WB, IHC (frozen)

• Field of Research:

  Signal Transduction

• Assay Principle:

  Detects Chx10 by Western blot at 0.5 to 1 µg/mL. Detects a 46 kDa band in mouse and rat retinal tissue lysates. Optimal concentration should be evaluated by serial dilutions. Suitable for use against recombinant proteins conjµgated to OVA, GST, His tags and other.

• Stability:

  See expiration date on vial

• Concentration:

  See vial for Concentration

• Form:

  Ammonium sµLphate precipitated antibody in PBS with 0,08% sodium azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. Liu, I.S., et al. Developmental expression of a novel murine homeobox gene (Chx10): evidence for roles in determination of the neuroretina and inner nuclear layer. Neuron 1994, 13, 377-393 _x000D_ _x000D_ 2. Chen, C.M. , Cepko, C.L. Expression of Chx10 and Chx10-1 in the developing chicken retina. Mech. Dev. 2000, 90, 293-297 _x000D_ _x000D_ 3. Ferda Percin, E., et al. Human microphthalmia associated with mutations in the retinal homeobox gene CHX10. Nat. Genet. 2000, 25, 397-401_x000D_ _x000D_ 4. Nittner, D., et al. Synthetic lethality between Rb, p53 and Dicer or miR-17–92 in retinal progenitors suppresses retinoblastoma formation. Nature Cell Biology (2012), 14, 958-965 _x000D_ 5. Kay, J.N., et al. Neurod6 expression defines new retinal amacrine cell subtypes and regulates their fate. Nature Neuroscience (2011), 14, 965-972_x000D_ 6. Eiraku, M., et al. Self-organizing optic-cup morphogenesis in three-dimensional culture. Nature (2011), 472, 51-56 7. Pierfelice, T.J., et al. Notch3 Activation Promotes Invasive Glioma Formation in a Tissue Site-Specific Manner. Cancer Res., 71, 1115-1125 (2011)._x000D_ _x000D_ 7. Takae Kiyama, Ching-Kang Chen, Steven W Wang, Ping Pan, Zhenlin Ju, Jing Wang, Shinako Takada, William H Klein, Chai-An Mao_x000D_ Essential roles of mitochondrial biogenesis regulator Nrf1 in retinal development and homeostasis_x000D_ https://doi.org/10.1186/s13024-018-0287-z_x000D_ _x000D_ 8. Anastasia Marie Litke; Sarah Samuelson; Kerry R. Delaney; Yves Sauvé; Robert L. Chow_x000D_ Investigating the Pathogenicity of VSX1 Missense Mutations and Their Association With Corneal Disease_x000D_ Investigative Ophthalmology , Visual Science December 2018, Vol.59, 5824-5835. doi:10.1167/iovs.18-2549

• Shipping Conditions:

  Ambient Temperature, freeze upon arrival

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• Functional Analysis:

  WB

• CAS Number:

  9007-83-4