Sheep anti Human TAK1

Sheep anti Human TAK1

• Background: C-TAK1 (Cdc25C associated protein kinase) is a ubiquitously expressed protein kinase found in Human tissues and cell lines and can phosphorylate Cdc25C on serine 216 in vitro. C-TAK1 is distinct from the DNA damage checkpoint kinase Chk1 and may regulate interactions between Cdc25C and 14-3-3 in vivo by phosphorylating Cdc25C at serine 216.
• Synonyms: Mark3, Sheep Anti-Cdc25c associated Protein kinase-1, Human
• CAS Number: 9007-83-4
• UniProt: P27448
• Host: Sheep
• Species Reactivity: Human
• Isotype: IgG
• Conjugation: Unconjugated
• Type: Polyclonal Antibody
• Applications: IP, WB
• Field of Research: Signal Transduction
• Purification Method: Ammonium Sulfate Precipitation
• Assay Principle: Antibody detects c-tak1 by Western blot analysis at 1 to 5 µg/mL. Detects c-tak1 in RKO and HCT116 cell lysates. Optimal concentration should be evaluated by serial dilutions.
• Stability: See expiration date on vial
• Concentration: See vial for Concentration
• Form: Provided as solution in phosphate buffered saline with 0,08% sodium azide
• Precautions: This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
• References & Citations: 1. Peng, C.Y., et al. C-TAK1 protein kinase phosphorylates Human Cdc25C on serine 216 and promotes 14-3-3 protein binding. Cell Growth Differ. 1998, 9, 197-208 2. Ogg, S., et al. Purification of a serine kinase that associates with and phosphorylates Human Cdc25C on serine 216. J. Biol. Chem. 1994, 269, 30461-30469 3. Busby, E.C., et al. The radiosensitizing agent 7-hydroxystaurosporine (UCN-01) inhibits the DNA damage checkpoint kinase hChk1. Cancer Res. 2000, 60, 2108-2112
• Shipping Conditions: Ambient Temperature, freeze upon arrival
• Storage Conditions: Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
• Functional Analysis: WB