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Recombinant Cdc20 Antibody

CAT: 0800-V9429SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V9429SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Cyclins, regulatory subunits, which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
Q12834
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human Cdc20 protein was used as the immunogen for the recombinant Cdc20 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RCDC20/7184
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Aliquot the recombinant Cdc20 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This recombinant Cdc20 antibody is available for research use only.
Storage Conditions
Aliquot the recombinant Cdc20 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the recombinant Cdc20 antibody should be determined by the researcher.
Location
Cytoplasm
Image Legend
IHC staining of FFPE human kidney cancer tissue with recombinant Cdc20 antibody (clone rCDC20/7184) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

UniProtKB · Q12834

Cell division cycle protein 20 homolog

CDC20_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q12834
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CDC20
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B2R6Z6, D3DPJ1, Q5JUY4, Q9BW56, Q9UQI9
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationIsopeptide bondPhosphoproteinUbl conjugation

Biological process

Cell cycleCell divisionDifferentiationMitosisNeurogenesisUbl conjugation pathway

Cellular component

CentromereChromosomeCytoplasmCytoskeletonKinetochore

Disease

Disease variant

Domain

RepeatWD repeat

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