Cdc20 Antibody

• Description:

  Cyclins, regulatory subunits, which associate with kinases, control many of the important steps in cell cycle progression. The Cdc2 protein kinase (p34Cdc2) exhibits protein kinase activity in vitro and exists in a complex with both cyclin B and a protein homologous to p13SUC1. Cdc2 kinase is the active subunit of the M phase promoting factor (MPF) and the M phase-specific Histone H1 kinase. The p34Cdc2/cyclin B complex is required for the G2 to M transition. An additional cell cycle-dependent protein kinase, termed p55cdc, exhibits a high degree of homology with the S. cerevisiae proteins Cdc20 and Cdc4. The p55cdc transcript is readily detectable in a variety of cultured cell lines in growth phase, but disappears when cell growth is chemically arrested.

• Specifications:

  The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• UniProt:

  Q12834

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant human protein was used as the immunogen for the Cdc20 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  CDC20/1102

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

• Reconstitution:

  Store the Cdc20 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Cdc20 antibody is available for research use only.

• Storage Conditions:

  Store the Cdc20 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  IHC: Formalin-fixed, paraffin-embedded human gastric carcinoma stained with Cdc20 antibody (CDC20/1102)