Recombinant Bcl-6 Antibody

• Description:

  Recognizes a protein of 95kDa, which is identified as Bcl-6. Antibody to bcl-6 is helpful in a number of diagnostic settings: (1) In the differential diagnosis of small B-cell lymphoma. Follicular lymphoma will show bcl-6 (and CD10) positivity whereas other small B-cell lymphomas are usually negative. (2) Bcl-6 is an important prognostic marker in diffuse large B-cell lymphomas (DLBCL), where CD10, bcl-6 and MUM1/IRF4 are used to identify germinal center and activated B-cell phenotypes. (3) Bcl-6 can be valuable in distinguishing classical Hodgkin lymphoma from nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) . The Reed-Sternberg cells of classical Hodgkin lymphoma are bcl-6 negative whereas the large (L&H) cells of NLPHL are bcl-6 positive. In contrast, anti-Bcl-6 rarely stains mantle-cell lymphoma and MALT lymphoma.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P41182

• Host:

  Rabbit

• Reactivity:

  Human

• Immunogen:

  A recombinant fragment (amino acids 1-400) of human BCL6 protein was used as the immunogen for the recombinant Bcl-6 antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  BCL6/9222R

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the recombinant Bcl-6 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This recombinant Bcl-6 antibody is available for research use only.

• Storage Conditions:

  Aliquot the recombinant Bcl-6 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the recombinant Bcl-6 antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Nucleus

• Image Legend:

  IHC staining of FFPE human tonsil tissue with recombinant Bcl-6 antibody (clone BCL6/9222R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.