PRMT7 Antibody / Protein arginine N-methyltransferase 7

• Description:

  Protein arginine methyltransferase 7 is a protein that in humans is encoded by the PRMT7 gene. This gene encodes a member of the protein arginine N-methyltransferase family of proteins. The encoded enzyme transfers single methyl groups to arginine residues to generate monomethylarginines on histone proteins as well as other protein substrates. This enzyme plays a role in a wide range of biological processes, including neuronal differentiation, male germ line imprinting, small nuclear ribonucleoprotein biogenesis, and regulation of the Wnt signaling pathway. Mutations in this gene underlie multiple related syndromes in human patients characterized by intellectual disability, short stature and other features. The encoded protein may promote breast cancer cell invasion and metastasis in human patients.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL, Immunofluorescence: 5 µg/mL

• UniProt:

  Q9NVM4

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  E. coli-derived recombinant human protein (amino acids K121-D526) was used as the immunogen for the PRMT7 antibody.

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Applications:

  WB, IHC-P, FACS, IF, Direct ELISA

• Purity:

  Antigen affinity purified

• Format:

  Antigen affinity purified

• Buffer:

  Lyophilized from 1X PBS with 2% Trehalose

• Reconstitution:

  After reconstitution, the PRMT7 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

• Limitations:

  This PRMT7 antibody is available for research use only.

• Storage Conditions:

  After reconstitution, the PRMT7 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

• Formulation:

  0.5 mg/mL if reconstituted with 0.2ml sterile DI water

• Applications Notes:

  Optimal dilution of the PRMT7 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nuclear, cytoplasmic

• Image Legend:

  IHC staining of FFPE mouse testis tissue with PRMT7 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.